FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2131056555> ?p ?o ?g. }

• W2131056555 abstract "ABSTRACT The human fungal pathogen Cryptococcus neoformans is capable of infecting a broad range of hosts, from invertebrates like amoebas and nematodes to standard vertebrate models such as mice and rabbits. Here we have taken advantage of a zebrafish model to investigate host-pathogen interactions of Cryptococcus with the zebrafish innate immune system, which shares a highly conserved framework with that of mammals. Through live-imaging observations and genetic knockdown, we establish that macrophages are the primary immune cells responsible for responding to and containing acute cryptococcal infections. By interrogating survival and cryptococcal burden following infection with a panel of Cryptococcus mutants, we find that virulence factors initially identified as important in causing disease in mice are also necessary for pathogenesis in zebrafish larvae. Live imaging of the cranial blood vessels of infected larvae reveals that C. neoformans is able to penetrate the zebrafish brain following intravenous infection. By studying a C. neoformans FNX1 gene mutant, we find that blood-brain barrier invasion is dependent on a known cryptococcal invasion-promoting pathway previously identified in a murine model of central nervous system invasion. The zebrafish- C. neoformans platform provides a visually and genetically accessible vertebrate model system for cryptococcal pathogenesis with many of the advantages of small invertebrates. This model is well suited for higher-throughput screening of mutants, mechanistic dissection of cryptococcal pathogenesis in live animals, and use in the evaluation of therapeutic agents. IMPORTANCE Cryptococcus neoformans is an important opportunistic pathogen that is estimated to be responsible for more than 600,000 deaths worldwide annually. Existing mammalian models of cryptococcal pathogenesis are costly, and the analysis of important pathogenic processes such as meningitis is laborious and remains a challenge to visualize. Conversely, although invertebrate models of cryptococcal infection allow high-throughput assays, they fail to replicate the anatomical complexity found in vertebrates and, specifically, cryptococcal stages of disease. Here we have utilized larval zebrafish as a platform that overcomes many of these limitations. We demonstrate that the pathogenesis of C. neoformans infection in zebrafish involves factors identical to those in mammalian and invertebrate infections. We then utilize the live-imaging capacity of zebrafish larvae to follow the progression of cryptococcal infection in real time and establish a relevant model of the critical central nervous system infection phase of disease in a nonmammalian model." @default.
• W2131056555 created "2016-06-24" @default.
• W2131056555 creator A5028777325 @default.
• W2131056555 creator A5055071986 @default.
• W2131056555 creator A5057736752 @default.
• W2131056555 creator A5061409450 @default.
• W2131056555 creator A5066781175 @default.
• W2131056555 date "2015-10-30" @default.
• W2131056555 modified "2023-10-02" @default.
• W2131056555 title "Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion" @default.
• W2131056555 cites W1490724351 @default.
• W2131056555 cites W1495151309 @default.
• W2131056555 cites W1583386719 @default.
• W2131056555 cites W1654353550 @default.
• W2131056555 cites W1986514504 @default.
• W2131056555 cites W2001430852 @default.
• W2131056555 cites W2005800004 @default.
• W2131056555 cites W2034064528 @default.
• W2131056555 cites W2039568446 @default.
• W2131056555 cites W2039830769 @default.
• W2131056555 cites W2040386323 @default.
• W2131056555 cites W2050195517 @default.
• W2131056555 cites W2053677632 @default.
• W2131056555 cites W2055004906 @default.
• W2131056555 cites W2055416073 @default.
• W2131056555 cites W2068175743 @default.
• W2131056555 cites W2076237697 @default.
• W2131056555 cites W2076970838 @default.
• W2131056555 cites W2079513681 @default.
• W2131056555 cites W2084080581 @default.
• W2131056555 cites W2085415570 @default.
• W2131056555 cites W2092074715 @default.
• W2131056555 cites W2093953341 @default.
• W2131056555 cites W2095601290 @default.
• W2131056555 cites W2098472248 @default.
• W2131056555 cites W2104048810 @default.
• W2131056555 cites W2105998029 @default.
• W2131056555 cites W2122002065 @default.
• W2131056555 cites W2136143654 @default.
• W2131056555 cites W2144671897 @default.
• W2131056555 cites W2148959625 @default.
• W2131056555 cites W2153202357 @default.
• W2131056555 cites W2155212464 @default.
• W2131056555 cites W2156654507 @default.
• W2131056555 cites W2158111073 @default.
• W2131056555 cites W2161415571 @default.
• W2131056555 cites W2162644900 @default.
• W2131056555 cites W2163444398 @default.
• W2131056555 cites W2165159573 @default.
• W2131056555 cites W2166353600 @default.
• W2131056555 doi "https://doi.org/10.1128/mbio.01425-15" @default.
• W2131056555 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4611042" @default.
• W2131056555 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26419880" @default.
• W2131056555 hasPublicationYear "2015" @default.
• W2131056555 type Work @default.
• W2131056555 sameAs 2131056555 @default.
• W2131056555 citedByCount "61" @default.
• W2131056555 countsByYear W21310565552016 @default.
• W2131056555 countsByYear W21310565552017 @default.
• W2131056555 countsByYear W21310565552018 @default.
• W2131056555 countsByYear W21310565552019 @default.
• W2131056555 countsByYear W21310565552020 @default.
• W2131056555 countsByYear W21310565552021 @default.
• W2131056555 countsByYear W21310565552022 @default.
• W2131056555 countsByYear W21310565552023 @default.
• W2131056555 crossrefType "journal-article" @default.
• W2131056555 hasAuthorship W2131056555A5028777325 @default.
• W2131056555 hasAuthorship W2131056555A5055071986 @default.
• W2131056555 hasAuthorship W2131056555A5057736752 @default.
• W2131056555 hasAuthorship W2131056555A5061409450 @default.
• W2131056555 hasAuthorship W2131056555A5066781175 @default.
• W2131056555 hasBestOaLocation W21310565551 @default.
• W2131056555 hasConcept C104317684 @default.
• W2131056555 hasConcept C136449434 @default.
• W2131056555 hasConcept C19843653 @default.
• W2131056555 hasConcept C203014093 @default.
• W2131056555 hasConcept C2776460866 @default.
• W2131056555 hasConcept C2776878037 @default.
• W2131056555 hasConcept C2777893358 @default.
• W2131056555 hasConcept C2778952914 @default.
• W2131056555 hasConcept C2779413141 @default.
• W2131056555 hasConcept C2780942790 @default.
• W2131056555 hasConcept C54355233 @default.
• W2131056555 hasConcept C60987743 @default.
• W2131056555 hasConcept C86803240 @default.
• W2131056555 hasConcept C8891405 @default.
• W2131056555 hasConcept C89423630 @default.
• W2131056555 hasConceptScore W2131056555C104317684 @default.
• W2131056555 hasConceptScore W2131056555C136449434 @default.
• W2131056555 hasConceptScore W2131056555C19843653 @default.
• W2131056555 hasConceptScore W2131056555C203014093 @default.
• W2131056555 hasConceptScore W2131056555C2776460866 @default.
• W2131056555 hasConceptScore W2131056555C2776878037 @default.
• W2131056555 hasConceptScore W2131056555C2777893358 @default.
• W2131056555 hasConceptScore W2131056555C2778952914 @default.
• W2131056555 hasConceptScore W2131056555C2779413141 @default.
• W2131056555 hasConceptScore W2131056555C2780942790 @default.
• W2131056555 hasConceptScore W2131056555C54355233 @default.
• W2131056555 hasConceptScore W2131056555C60987743 @default.
• W2131056555 hasConceptScore W2131056555C86803240 @default.

• next