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• W2131243604 abstract "2′-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical synthesis of such oligonucleotides is described. Two alternative approaches have been developed, one of which proceeds via a fully protected phosphoramidite of 5-fluoro-4-methylmercapto-2′-deoxyuridine 2, the other via a fully protected phosphoramidite of 5-fluoro-2′-deoxycytidine 3. Either building block can be used in automated oligonucleotide synthesis applying standard elongation cycles and deprotection procedures exclusively. The methylmercapto function of 2 is replaced by an amino group in the final ammonia treatment used for cleavage from support and base deprotection." @default.
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• W2131243604 date "1992-01-01" @default.
• W2131243604 modified "2023-10-18" @default.
• W2131243604 title "Chemical synthesis of 2′-deoxyoligonucleotides containing 5-fluoro-2′-deoxycytidine" @default.
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• W2131243604 doi "https://doi.org/10.1093/nar/20.10.2421" @default.
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