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- W2131293314 abstract "We designed a detection system of 5-methylcytosine (mC) in DNA by a combination of photooxidative DNA cleavage reaction of 2-methyl-1,4-naphthoquinone chromophore with invader technology. Enzymatic treatment of a mixture of photochemically fragmented target oligodeoxynucleotides (ODNs) at mC and probe oligomer possessing a fluorophore and a quencher resulted in a dramatic enhancement of fluorescence. In contrast, fluorescence emission for the ODN containing C but not mC at the target sequence was extremely weak. We could detect mC in sub-femtomol DNA by monitoring the fluorescence change." @default.
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- W2131293314 date "2006-11-01" @default.
- W2131293314 modified "2023-09-26" @default.
- W2131293314 title "Sensitive discrimination between cytosine and 5-methylcytosine in DNA by a modified invader method" @default.
- W2131293314 doi "https://doi.org/10.1093/nass/nrl081" @default.
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