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• W2131362342 abstract "In this paper, a new application of the hyper-Rayleigh scattering technique in determining multiple binding constants of a small molecule like bilirubin to a macromolecule like the protein human serum albumin has been demonstrated. Human serum albumin has two binding sites for bilirubin, and the binding constants have been measured by carrying out a second harmonic titration of the protein against bilirubin and vice versa. The measured binding constants K1 = 1.5 ± 0.43 × 107 M-1 and K2 = 1.01 ± 0.16 × 106 M-1 agree well with the reported values obtained by other methods." @default.
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• W2131362342 date "2005-03-01" @default.
• W2131362342 modified "2023-09-27" @default.
• W2131362342 title "Binding Constant Measurement by Hyper-Rayleigh Scattering: Bilirubin−Human Serum Albumin Binding as a Case Study" @default.
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• W2131362342 doi "https://doi.org/10.1021/jp047268n" @default.
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