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- W2131652014 abstract "A novel actinomycete strain, designated CS5-AC15 T , was isolated from a soil sample collected from a cliff on Mara Island, Jeju, Republic of Korea, and subjected to a polyphasic taxonomic analysis. The isolate produced well-developed, yellow substrate mycelium and white aerial mycelium that differentiated into straight or flexuous chains of smooth-surfaced spores. 16S rRNA gene sequence analyses showed that the organism belonged to the family Thermomonosporaceae and formed a tight cluster with the type strain of Actinomadura oligospora (97.4 % sequence similarity). Chemotaxonomic characteristics were consistent with its assignment to the genus Actinomadura in that the isolate had meso -diaminopimelic acid as the diagnostic diamino acid in the cell wall, madurose as the characteristic sugar, N -acetyl type of murein in the peptidoglycan, MK-9(H 6 ) and MK-9(H 8 ) as major menaquinones and a polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids. Mycolic acids were not detected. The predominant fatty acids were C 16 : 0 , C 18 : 1 ω9 c and iso-C 16 : 0 . The DNA G+C content was 70.9 mol%. DNA relatedness of strain CS5-AC15 T and A. oligospora JCM 10648 T was 37.9±0.7 %. On the basis of the phenotypic, phylogenetic and DNA–DNA hybridization data, strain CS5-AC15 T is assigned to a novel species of the genus Actinomadura , for which the name Actinomadura rupiterrae sp. nov. is proposed (type strain CS5-AC15 T = KCTC 19559 T = DSM 45251 T )." @default.
- W2131652014 created "2016-06-24" @default.
- W2131652014 creator A5091044844 @default.
- W2131652014 date "2012-04-01" @default.
- W2131652014 modified "2023-09-24" @default.
- W2131652014 title "Actinomadura rupiterrae sp. nov., isolated from cliff soil" @default.
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- W2131652014 doi "https://doi.org/10.1099/ijs.0.035121-0" @default.
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