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- W2131781180 abstract "Colonies of Bacillus anthracis Sterne allow the growth of papillation after 6 days of incubation at 30°C on Luria–Bertani medium. The papillae are due to mutations that allow the cells to overcome the barriers to continued growth. Cells isolated from papillae display two distinct gross phenotypes (group A and group B). We determined that group A mutants have mutations in the nprR gene including frameshifts, deletions, duplications and base substitutions. We used papillation as a tool for finding new mutators as the mutators generate elevated levels of papillation. We discovered that disruption of yycJ or recJ leads to a spontaneous mutator phenotype. We defined the nprR/papillation system as a new mutational analysis system for B. anthracis. The mutational specificity of the new mutator yycJ is similar to that of mismatch repair-deficient strains (MMR-) such as those with mutations in mutL or mutS. Deficiency in recJ results in a unique specificity, generating only tandem duplications." @default.
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- W2131781180 date "2011-01-11" @default.
- W2131781180 modified "2023-09-27" @default.
- W2131781180 title "Papillation in Bacillus anthracis colonies: a tool for finding new mutators" @default.
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- W2131781180 doi "https://doi.org/10.1111/j.1365-2958.2011.07519.x" @default.
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