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- W2131811477 abstract "The reversibility of hyperactivated motility was tested in caudal epididymal mouse sperm by treating them with 1 μM calcium ionophore A23187 in dimethyl sulfoxide (DMSO), followed 2 min later by the addition of medium containing high levels of bovine serum albumin (BSA) (final concetrations: 0.5 μM A23187, 22 mg/ml BSA). Controls received DMSO alone, followed by BSA. Immediately following treatment with A23187, motility was weak and vibratory. Two minutes after the addition of high levels of BSA, motility was hyperactivated, as determined by videotape analysis of linearity of trajectory and acuteness of flagellar bending. Ten minutes after the addition, the movement pattern returned to that of fresh, uncapacitated epididymal sperm. Control sperm retained the linear swimming pattern of fresh caudal epididymal sperm during the 10 min of observation. Ninety minutes later, however, both control and treated sperm became hyperactivated. The percentage of motile sperm was not affected by treatment or time. Thus, ionophoreinduced hyperactivation is reversible and does not interfere with the normal development of hyperactivation during incubation under capacitating conditions in vitro." @default.
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- W2131811477 date "1987-11-01" @default.
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- W2131811477 title "Hyperactivated motility induced in mouse sperm by calcium ionophore A23187 is reversible" @default.
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- W2131811477 doi "https://doi.org/10.1002/jez.1402440218" @default.
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