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- W2131844243 abstract "This paper presents the details of direct antigen coating (DAC) - ELISA and Dot-blot-ELISA for the detection of banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV) in banana leaf and pseudostem tissues. Suckers were collected from banana plants infected with BSV and CMV. The plants were indexed for presence of viruses by DAC-ELISA. The DAC form of indirect ELISA was adopted to detect viruses in plants. From the studies, it is observed that BSV and CMV induced similar interveinal chlorotic streaks of varied sizes in banana. In DAC-ELISA, BSV was detected up to 10 -2 dilutions of tissue extracts but it was detected by Dot-blot-ELISA up to 10 -3 dilution. Further, it is observed that among ten field collected samples, none were positive for CMV in DAC-ELISA, but three samples reacted positively in Dot-blot-ELISA. Out of ten field samples tested for BSV, only one weakly reacted. However, in Dot- blot-ELISA, none were found positive for BSV. Out of ten samples, none were found mixedly infected. Above findings indicate that Dot-blot-ELISA is relatively more sensitive for the detection of BSV and CMV in banana. (The Journal of American Science. 2008;4(2):49-57). (ISSN 1545-1003)." @default.
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- W2131844243 date "2008-01-01" @default.
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- W2131844243 title "Comparison Of Dac-Elisa And Dot-Blot-Elisa For The Detection Of Cucumber Mosaic And Banana Streak Viruses Infecting Banana" @default.
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