Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132025393> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2132025393 endingPage "S97" @default.
- W2132025393 startingPage "S94" @default.
- W2132025393 abstract "To elucidate the effects of high temperatures, wheat plants (Triticum aestivum cv. CPAN 1676) were given heat shocks at 37°C and 42°C for two hours, and responsive genes were identified through PCR-Select Subtraction technology. Four subtractive cDNA libraries, including three forward and one reverse subtraction, were constructed from three different developmental stages. A total of 5500 ESTs were generated and 3516 high quality ESTs were submitted to Genbank. More than one third of the ESTs generated fall in unknown/no hit categories upon a homology search through BLAST analysis. A large number of high temperature responsive genes have been identified and characterized. Reverse subtraction analysis in developing grains showed extensive transcriptional changes upon heat stress as revealed by comparative analysis with forward subtraction. Differential expression was confirmed by cDNA macroarray and by northern/RT-PCR analysis. Expression analysis of wheat plants subjected to high temperature stress, after one and four days of recovery, showed fast recovery in seedling tissues. However, recovery was small in the developing seed tissue after two hours of heat stress. Ten selected genes were analysed in further detail by quantitative real-time PCR in an array of 35 different wheat tissues representing major developmental stages as well as different abiotic stresses. Tissue specificity was examined along with cross talk with other abiotic stresses and putative signalling molecules. The results obtained contribute towards understanding the regulation of genes at different developmental stages in wheat crucial to withstanding and recovery from heat stress." @default.
- W2132025393 created "2016-06-24" @default.
- W2132025393 creator A5022149848 @default.
- W2132025393 creator A5023410431 @default.
- W2132025393 creator A5030662254 @default.
- W2132025393 date "2011-12-31" @default.
- W2132025393 modified "2023-10-18" @default.
- W2132025393 title "Characterization and expression of high temperature stress responsive genes in bread wheat (Triticum aestivum L.)" @default.
- W2132025393 cites W1966474554 @default.
- W2132025393 cites W1999177659 @default.
- W2132025393 cites W2018931916 @default.
- W2132025393 cites W2062377624 @default.
- W2132025393 cites W2116189858 @default.
- W2132025393 cites W2142303642 @default.
- W2132025393 cites W2158714788 @default.
- W2132025393 cites W2160030559 @default.
- W2132025393 cites W2207832617 @default.
- W2132025393 doi "https://doi.org/10.17221/3261-cjgpb" @default.
- W2132025393 hasPublicationYear "2011" @default.
- W2132025393 type Work @default.
- W2132025393 sameAs 2132025393 @default.
- W2132025393 citedByCount "8" @default.
- W2132025393 countsByYear W21320253932012 @default.
- W2132025393 countsByYear W21320253932014 @default.
- W2132025393 countsByYear W21320253932015 @default.
- W2132025393 countsByYear W21320253932017 @default.
- W2132025393 countsByYear W21320253932019 @default.
- W2132025393 countsByYear W21320253932020 @default.
- W2132025393 countsByYear W21320253932021 @default.
- W2132025393 crossrefType "journal-article" @default.
- W2132025393 hasAuthorship W2132025393A5022149848 @default.
- W2132025393 hasAuthorship W2132025393A5023410431 @default.
- W2132025393 hasAuthorship W2132025393A5030662254 @default.
- W2132025393 hasBestOaLocation W21320253931 @default.
- W2132025393 hasConcept C104317684 @default.
- W2132025393 hasConcept C132215390 @default.
- W2132025393 hasConcept C149034497 @default.
- W2132025393 hasConcept C150194340 @default.
- W2132025393 hasConcept C151730666 @default.
- W2132025393 hasConcept C187882448 @default.
- W2132025393 hasConcept C189482833 @default.
- W2132025393 hasConcept C196436469 @default.
- W2132025393 hasConcept C54355233 @default.
- W2132025393 hasConcept C79029880 @default.
- W2132025393 hasConcept C86803240 @default.
- W2132025393 hasConcept C89519541 @default.
- W2132025393 hasConceptScore W2132025393C104317684 @default.
- W2132025393 hasConceptScore W2132025393C132215390 @default.
- W2132025393 hasConceptScore W2132025393C149034497 @default.
- W2132025393 hasConceptScore W2132025393C150194340 @default.
- W2132025393 hasConceptScore W2132025393C151730666 @default.
- W2132025393 hasConceptScore W2132025393C187882448 @default.
- W2132025393 hasConceptScore W2132025393C189482833 @default.
- W2132025393 hasConceptScore W2132025393C196436469 @default.
- W2132025393 hasConceptScore W2132025393C54355233 @default.
- W2132025393 hasConceptScore W2132025393C79029880 @default.
- W2132025393 hasConceptScore W2132025393C86803240 @default.
- W2132025393 hasConceptScore W2132025393C89519541 @default.
- W2132025393 hasIssue "Special Issue" @default.
- W2132025393 hasLocation W21320253931 @default.
- W2132025393 hasOpenAccess W2132025393 @default.
- W2132025393 hasPrimaryLocation W21320253931 @default.
- W2132025393 hasRelatedWork W1464584225 @default.
- W2132025393 hasRelatedWork W1531528770 @default.
- W2132025393 hasRelatedWork W1997144223 @default.
- W2132025393 hasRelatedWork W2372998473 @default.
- W2132025393 hasRelatedWork W2384320101 @default.
- W2132025393 hasRelatedWork W2404499195 @default.
- W2132025393 hasRelatedWork W262379139 @default.
- W2132025393 hasRelatedWork W3148398443 @default.
- W2132025393 hasRelatedWork W3149400858 @default.
- W2132025393 hasRelatedWork W886888406 @default.
- W2132025393 hasVolume "47" @default.
- W2132025393 isParatext "false" @default.
- W2132025393 isRetracted "false" @default.
- W2132025393 magId "2132025393" @default.
- W2132025393 workType "article" @default.