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- W2132061714 endingPage "2036" @default.
- W2132061714 startingPage "2023" @default.
- W2132061714 abstract "The application of next-generation sequencing (NGS) to characterize cancer genomes has resulted in the discovery of numerous genetic markers. Consequently, the number of markers that warrant routine screening in molecular diagnostic laboratories, often from limited tumor material, has increased. This increased demand has been difficult to manage by traditional low- and/or medium-throughput sequencing platforms. Massively parallel sequencing capabilities of NGS provide a much-needed alternative for mutation screening in multiple genes with a single low investment of DNA. However, implementation of NGS technologies, most of which are for research use only (RUO), in a diagnostic laboratory, needs extensive validation in order to establish Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP)-compliant performance characteristics. Here, we have reviewed approaches for validation of NGS technology for routine screening of tumors. We discuss the criteria for selecting gene markers to include in the NGS panel and the deciding factors for selecting target capture approaches and sequencing platforms. We also discuss challenges in result reporting, storage and retrieval of the voluminous sequencing data and the future potential of clinical NGS." @default.
- W2132061714 created "2016-06-24" @default.
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- W2132061714 date "2015-10-14" @default.
- W2132061714 modified "2023-09-25" @default.
- W2132061714 title "Next-Generation Sequencing in Clinical Molecular Diagnostics of Cancer: Advantages and Challenges" @default.
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- W2132061714 doi "https://doi.org/10.3390/cancers7040874" @default.
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