FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132133886> ?p ?o ?g. }

• W2132133886 endingPage "76" @default.
• W2132133886 startingPage "69" @default.
• W2132133886 abstract "In this study, the developmental capacity and cytogenetic composition of different oocyte activation protocols was evaluated following intracytoplasmic sperm injection (ICSI) of in vitro matured bovine oocytes. Motile spermatozoa selected by Percoll density gradient were treated with 5 mM dithiothreitol (DTT) and analysed for ultrastructural changes of the head using transmission electron microscopy (TEM). The alterations in sperm morphology after DTT treatment for different times (15, 30 and 60 min) were 10%, 45-55% and 70-85%, respectively. Further, a partial decondensation of sperm heads was observed after DTT treatment for 30 min. Oocytes were injected with sperm treated with DTT for 30 min. In group 1, sperm injection was performed without any activation stimulus to the oocytes. In group 2, sham injection without sperm was performed without activating the oocytes. Oocytes injected with sperm exposed to 5 μM ionomycin for 5 min (group 3), 5 μM ionomycin + 1.9 mM dimethylaminopurine (DMAP) for 3 h (group 4) and 5 µM ionomycin + 3 h culture in M199 + 1.9 mM DMAP (group 5) were also evaluated for cleavage, development and chromosomal abnormality. Cleavage and development rates in groups 1, 2 and 3 were significantly ( p < 0.05) lower than those in groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycin (group 4) was higher than in group 5. We conclude that immediate DMAP treatment after ionomycin exposure of oocytes results in arrest of release of the second polar body, and thus leads to changes in chromosomal pattern. Therefore, the time interval between ionomycin and DMAP plays a crucial role in bovine ICSI." @default.
• W2132133886 created "2016-06-24" @default.
• W2132133886 creator A5018250506 @default.
• W2132133886 creator A5047662117 @default.
• W2132133886 creator A5050935020 @default.
• W2132133886 creator A5054365406 @default.
• W2132133886 creator A5062416696 @default.
• W2132133886 creator A5090719390 @default.
• W2132133886 date "2003-02-01" @default.
• W2132133886 modified "2023-09-30" @default.
• W2132133886 title "Different activation treatments for successful development of bovine oocytes following intracytoplasmic sperm injection" @default.
• W2132133886 cites W1976269625 @default.
• W2132133886 cites W1985104418 @default.
• W2132133886 cites W1985426653 @default.
• W2132133886 cites W1991682148 @default.
• W2132133886 cites W2004684403 @default.
• W2132133886 cites W2005004020 @default.
• W2132133886 cites W2018221132 @default.
• W2132133886 cites W2020275692 @default.
• W2132133886 cites W2023665378 @default.
• W2132133886 cites W2042447008 @default.
• W2132133886 cites W2061064038 @default.
• W2132133886 cites W2061759606 @default.
• W2132133886 cites W2075820401 @default.
• W2132133886 cites W2076579161 @default.
• W2132133886 cites W2088778898 @default.
• W2132133886 cites W2099964328 @default.
• W2132133886 cites W2128109524 @default.
• W2132133886 cites W2150869497 @default.
• W2132133886 cites W2158119378 @default.
• W2132133886 cites W2345672546 @default.
• W2132133886 cites W2040073329 @default.
• W2132133886 doi "https://doi.org/10.1017/s0967199403001096" @default.
• W2132133886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12625531" @default.
• W2132133886 hasPublicationYear "2003" @default.
• W2132133886 type Work @default.
• W2132133886 sameAs 2132133886 @default.
• W2132133886 citedByCount "34" @default.
• W2132133886 countsByYear W21321338862012 @default.
• W2132133886 countsByYear W21321338862013 @default.
• W2132133886 countsByYear W21321338862014 @default.
• W2132133886 countsByYear W21321338862016 @default.
• W2132133886 countsByYear W21321338862017 @default.
• W2132133886 countsByYear W21321338862018 @default.
• W2132133886 countsByYear W21321338862019 @default.
• W2132133886 countsByYear W21321338862020 @default.
• W2132133886 countsByYear W21321338862021 @default.
• W2132133886 countsByYear W21321338862022 @default.
• W2132133886 crossrefType "journal-article" @default.
• W2132133886 hasAuthorship W2132133886A5018250506 @default.
• W2132133886 hasAuthorship W2132133886A5047662117 @default.
• W2132133886 hasAuthorship W2132133886A5050935020 @default.
• W2132133886 hasAuthorship W2132133886A5054365406 @default.
• W2132133886 hasAuthorship W2132133886A5062416696 @default.
• W2132133886 hasAuthorship W2132133886A5090719390 @default.
• W2132133886 hasConcept C16685009 @default.
• W2132133886 hasConcept C179265757 @default.
• W2132133886 hasConcept C181199279 @default.
• W2132133886 hasConcept C185592680 @default.
• W2132133886 hasConcept C196843134 @default.
• W2132133886 hasConcept C202751555 @default.
• W2132133886 hasConcept C2776690073 @default.
• W2132133886 hasConcept C2776989580 @default.
• W2132133886 hasConcept C2777824588 @default.
• W2132133886 hasConcept C2778278907 @default.
• W2132133886 hasConcept C2781087480 @default.
• W2132133886 hasConcept C2910992960 @default.
• W2132133886 hasConcept C512716672 @default.
• W2132133886 hasConcept C55493867 @default.
• W2132133886 hasConcept C71924100 @default.
• W2132133886 hasConcept C86803240 @default.
• W2132133886 hasConcept C95444343 @default.
• W2132133886 hasConceptScore W2132133886C16685009 @default.
• W2132133886 hasConceptScore W2132133886C179265757 @default.
• W2132133886 hasConceptScore W2132133886C181199279 @default.
• W2132133886 hasConceptScore W2132133886C185592680 @default.
• W2132133886 hasConceptScore W2132133886C196843134 @default.
• W2132133886 hasConceptScore W2132133886C202751555 @default.
• W2132133886 hasConceptScore W2132133886C2776690073 @default.
• W2132133886 hasConceptScore W2132133886C2776989580 @default.
• W2132133886 hasConceptScore W2132133886C2777824588 @default.
• W2132133886 hasConceptScore W2132133886C2778278907 @default.
• W2132133886 hasConceptScore W2132133886C2781087480 @default.
• W2132133886 hasConceptScore W2132133886C2910992960 @default.
• W2132133886 hasConceptScore W2132133886C512716672 @default.
• W2132133886 hasConceptScore W2132133886C55493867 @default.
• W2132133886 hasConceptScore W2132133886C71924100 @default.
• W2132133886 hasConceptScore W2132133886C86803240 @default.
• W2132133886 hasConceptScore W2132133886C95444343 @default.
• W2132133886 hasIssue "1" @default.
• W2132133886 hasLocation W21321338861 @default.
• W2132133886 hasLocation W21321338862 @default.
• W2132133886 hasOpenAccess W2132133886 @default.
• W2132133886 hasPrimaryLocation W21321338861 @default.
• W2132133886 hasRelatedWork W1972453963 @default.
• W2132133886 hasRelatedWork W2045299582 @default.
• W2132133886 hasRelatedWork W2061759606 @default.
• W2132133886 hasRelatedWork W2130263981 @default.

• next