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- W2132182017 abstract "Cellobiohydrolases (EC 3.2.1.91) are important exo enzymes involved in cellulose hydrolysis alongside endoglucanases (EC 3.2.1.4) and βglucosidases (EC 3.2.1.21). Heterologous cellobiohydrolase gene expression under constitutive promoter control using Saccharomyces cerevisiae as host system is of great importance for a successful SSF process. From this point of view, the main objective of the work was to use Yeplac181 expression vector as a recipient for cellobiohdrolase cbhB encoding gene expression under the control of the actin promoter, in Saccharomyces cerevisiae. Two hybrid vectors, YEplac-Actp and YEplac-Actp-CbhB, were generated using Escherichia coli XLI Blue for the cloning experiments. Constitutive cbhB gene expression was checked by proteine gel electrophoresis (SDS-PAGE) after insertion of these constructs into Saccharomyces cerevisiae." @default.
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- W2132182017 date "2012-01-01" @default.
- W2132182017 modified "2023-09-26" @default.
- W2132182017 title "Recombinant vectors construction for cellobiohydrolase encoding gene constitutive expression." @default.
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