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- W2132208519 abstract "An immunocytochemical staining method for light and electron microscopy was developed to permit adequate penetration of staining conjugates with high specificity, while preserving acceptable ultrastructure. For this purpose an indirect immunoperoxidase method with Staphylococcal protein A-peroxidase conjugates was used in the presence of saponin on aldehyde-saponin-fixed cells. As the first application, fibronectin was localized intracellularly in human embryonic skin fibroblasts. Fibronectin was detected in large amounts in the cisternae of rough endoplasmic reticulum and in 200 nm (secretory?) vesicles. Little fibronectin was present in the Golgi complex; the stacked Golgi cisternae were conspicuously devoid of this protein. The 200 nm vesicles were mostly distributed on the mature side of the Golgi apparatus. These results indicate that fibronectin is exclusively localized to intracellular structures involved in secretory function and suggest that fibronectin may not be processed in significant amounts within the cisternal stacks of the Golgi complex." @default.
- W2132208519 created "2016-06-24" @default.
- W2132208519 creator A5006232448 @default.
- W2132208519 date "1980-11-01" @default.
- W2132208519 modified "2023-10-17" @default.
- W2132208519 title "Intracellular localization of fibronectin using immunoperoxidase cytochemistry in light and electron microscopy." @default.
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- W2132208519 doi "https://doi.org/10.1177/28.11.7000891" @default.
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