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- W2132227251 abstract "A capture enzyme immunoassay was developed for the detection of Mycoplasma bovis antigens in bull semen or preputial washings. IgG prepared from rabbits immunised with M bovis was passively adsorped to 96-well polystyrene plates. This antibody captured M bovis antigens which were then detected by using an IgG preparation from an immunised cow and murine monoclonal antibody to the bovine L-chain conjugated with horseradish peroxidase. The sensitivity of the assay was approximately 200 colour changing units (ccu)/ml and the specificity was excellent in that other species of mycoplasma, ureaplasma or acholeplasma did not react. A blind study of bull semen experimentally contaminated with M bovis detected all specimens with more than 200 ccu/ml." @default.
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- W2132227251 date "1987-06-20" @default.
- W2132227251 modified "2023-09-23" @default.
- W2132227251 title "Enzyme immunoassay for detection of Mycoplasma bovis antigens in bull semen and preputial washings" @default.
- W2132227251 doi "https://doi.org/10.1136/vr.120.25.596" @default.
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