Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132244902> ?p ?o ?g. }
- W2132244902 abstract "The activity-regulated gene expression of transcription factors is required for neural plasticity and function in response to neuronal stimulation. T-brain-1 (TBR1), a critical neuron-specific transcription factor for forebrain development, has been recognized as a high-confidence risk gene for autism spectrum disorders. Here, we show that in addition to its role in brain development, Tbr1 responds to neuronal activation and further modulates the Grin2b expression in adult brains and mature neurons. The expression levels of Tbr1 were investigated using both immunostaining and quantitative reverse transcription polymerase chain reaction (RT-PCR) analyses. We found that the mRNA and protein expression levels of Tbr1 are induced by excitatory synaptic transmission driven by bicuculline or glutamate treatment in cultured mature neurons. The upregulation of Tbr1 expression requires the activation of both α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors. Furthermore, behavioral training triggers Tbr1 induction in the adult mouse brain. The elevation of Tbr1 expression is associated with Grin2b upregulation in both mature neurons and adult brains. Using Tbr1-deficient neurons, we further demonstrated that TBR1 is required for the induction of Grin2b upon neuronal activation. Taken together with the previous studies showing that TBR1 binds the Grin2b promoter and controls expression of luciferase reporter driven by Grin2b promoter, the evidence suggests that TBR1 directly controls Grin2b expression in mature neurons. We also found that the addition of the calcium/calmodulin-dependent protein kinase II (CaMKII) antagonist KN-93, but not the calcium-dependent phosphatase calcineurin antagonist cyclosporin A, to cultured mature neurons noticeably inhibited Tbr1 induction, indicating that neuronal activation upregulates Tbr1 expression in a CaMKII-dependent manner. In conclusion, our study suggests that Tbr1 plays an important role in adult mouse brains in response to neuronal activation to modulate the activity-regulated gene transcription required for neural plasticity." @default.
- W2132244902 created "2016-06-24" @default.
- W2132244902 creator A5027224164 @default.
- W2132244902 creator A5055749746 @default.
- W2132244902 creator A5056699917 @default.
- W2132244902 date "2014-09-10" @default.
- W2132244902 modified "2023-09-29" @default.
- W2132244902 title "Neuronal excitation upregulates Tbr1, a high-confidence risk gene of autism, mediating Grin2b expression in the adult brain" @default.
- W2132244902 cites W1541267199 @default.
- W2132244902 cites W1624747402 @default.
- W2132244902 cites W1898303357 @default.
- W2132244902 cites W1964444099 @default.
- W2132244902 cites W1969358413 @default.
- W2132244902 cites W1970748319 @default.
- W2132244902 cites W1971095698 @default.
- W2132244902 cites W1973138133 @default.
- W2132244902 cites W1984199947 @default.
- W2132244902 cites W1985591145 @default.
- W2132244902 cites W1985661446 @default.
- W2132244902 cites W1988670106 @default.
- W2132244902 cites W1990500865 @default.
- W2132244902 cites W1999147940 @default.
- W2132244902 cites W2001746980 @default.
- W2132244902 cites W2004159036 @default.
- W2132244902 cites W2004784342 @default.
- W2132244902 cites W2009029265 @default.
- W2132244902 cites W2014042333 @default.
- W2132244902 cites W2016532227 @default.
- W2132244902 cites W2018419725 @default.
- W2132244902 cites W2024469079 @default.
- W2132244902 cites W2024663595 @default.
- W2132244902 cites W2027795629 @default.
- W2132244902 cites W2029245198 @default.
- W2132244902 cites W2030070790 @default.
- W2132244902 cites W2030930681 @default.
- W2132244902 cites W2031598294 @default.
- W2132244902 cites W2033606638 @default.
- W2132244902 cites W2035550082 @default.
- W2132244902 cites W2035857873 @default.
- W2132244902 cites W2043006474 @default.
- W2132244902 cites W2049483389 @default.
- W2132244902 cites W2052065200 @default.
- W2132244902 cites W2053938654 @default.
- W2132244902 cites W2054170041 @default.
- W2132244902 cites W2055314083 @default.
- W2132244902 cites W2056013470 @default.
- W2132244902 cites W2058877345 @default.
- W2132244902 cites W2059208184 @default.
- W2132244902 cites W2060688655 @default.
- W2132244902 cites W2061600370 @default.
- W2132244902 cites W2067395837 @default.
- W2132244902 cites W2074076965 @default.
- W2132244902 cites W2075725530 @default.
- W2132244902 cites W2081070804 @default.
- W2132244902 cites W2088350638 @default.
- W2132244902 cites W2089006095 @default.
- W2132244902 cites W2089397711 @default.
- W2132244902 cites W2090719035 @default.
- W2132244902 cites W2091347201 @default.
- W2132244902 cites W2106726734 @default.
- W2132244902 cites W2108881810 @default.
- W2132244902 cites W2109726419 @default.
- W2132244902 cites W2109807917 @default.
- W2132244902 cites W2115736681 @default.
- W2132244902 cites W2116629441 @default.
- W2132244902 cites W2122148359 @default.
- W2132244902 cites W2128493759 @default.
- W2132244902 cites W2132486539 @default.
- W2132244902 cites W2143302994 @default.
- W2132244902 cites W2146727059 @default.
- W2132244902 cites W2152231606 @default.
- W2132244902 cites W2170083890 @default.
- W2132244902 doi "https://doi.org/10.3389/fncel.2014.00280" @default.
- W2132244902 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4159980" @default.
- W2132244902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25309323" @default.
- W2132244902 hasPublicationYear "2014" @default.
- W2132244902 type Work @default.
- W2132244902 sameAs 2132244902 @default.
- W2132244902 citedByCount "42" @default.
- W2132244902 countsByYear W21322449022015 @default.
- W2132244902 countsByYear W21322449022016 @default.
- W2132244902 countsByYear W21322449022017 @default.
- W2132244902 countsByYear W21322449022018 @default.
- W2132244902 countsByYear W21322449022019 @default.
- W2132244902 countsByYear W21322449022020 @default.
- W2132244902 countsByYear W21322449022021 @default.
- W2132244902 countsByYear W21322449022022 @default.
- W2132244902 countsByYear W21322449022023 @default.
- W2132244902 crossrefType "journal-article" @default.
- W2132244902 hasAuthorship W2132244902A5027224164 @default.
- W2132244902 hasAuthorship W2132244902A5055749746 @default.
- W2132244902 hasAuthorship W2132244902A5056699917 @default.
- W2132244902 hasBestOaLocation W21322449021 @default.
- W2132244902 hasConcept C104317684 @default.
- W2132244902 hasConcept C126322002 @default.
- W2132244902 hasConcept C127561419 @default.
- W2132244902 hasConcept C134018914 @default.
- W2132244902 hasConcept C33594762 @default.
- W2132244902 hasConcept C55493867 @default.
- W2132244902 hasConcept C71924100 @default.