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• W2132313105 abstract "ABSTRACT Background: How HSV-1 DNA replication is initiated in infected cells is not fully understood. Experiments with temperature-sensitive HSV mutants have shown that DNA replication is a biphasic process that initially depends on the origin binding protein. Aims: The aim of the study was to answer the question at which origin of replication the HSV-1 DNA replication starts in the infected cell. Methods: Using the tsS mutant the HSV-1 infection was synchronized and newly synthesized nascent DNA fragments were analysed. Results: Nascent viral DNA was observed predominantly around the oriL, giving raise to the hypothesis that the replication starts at this origin in vivo. Conclusion: We show for the first time that HSV-1 DNA replication begins exclusively at the oriL site and proceeds in a bidirectional manner." @default.
• W2132313105 created "2016-06-24" @default.
• W2132313105 creator A5045180429 @default.
• W2132313105 creator A5080317817 @default.
• W2132313105 date "2015-03-01" @default.
• W2132313105 modified "2023-10-16" @default.
• W2132313105 title "Isolation of nascent DNA fragments from cells synchronously infected with HSV-1 reveals bidirectional initiation of replication at oriL" @default.
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• W2132313105 doi "https://doi.org/10.2217/fvl.14.112" @default.
• W2132313105 hasPublicationYear "2015" @default.
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