FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132320427> ?p ?o ?g. }

• W2132320427 endingPage "339" @default.
• W2132320427 startingPage "329" @default.
• W2132320427 abstract "The transport of proteins into the nucleus is a receptor-mediated process that is likely to involve between 50-100 gene products, including many that comprise the nuclear pore complex. We have developed an assay in Saccharomyces cerevisiae for the nuclear transport of green fluorescent protein fused to the SV-40 large T antigen nuclear localization signal (NLS-GFP). This assay allows the measurement of relative NLS-GFP nuclear import rates in wild-type and mutant cells under various physiological conditions. Probably the best understood component of the nuclear transport apparatus is Srp1p, the NLS receptor, which binds NLS-cargo in the cytoplasm and accompanies it into the nucleus. When compared to SRP1+ cells, NLS-GFP import rates in temperature-sensitive srp1-31 cells were slower and showed a lower temperature optimum. The in vivo transport defect of the srp1-31 cells was correlated with the purified protein's thermal sensitivity, as assayed by in vitro NLS peptide binding. We show that the kinetics of NLS-directed nuclear transport in wild-type cells is stimulated by the elevated expression of SSA1, which encodes a cytoplasmic heat shock protein 70 (Hsp70). Elevated Hsp70 levels are sufficient to suppress the NLS-GFP import defects in srp1-31 and nup82-3 cells. NUP82 encodes a protein that functions within the nuclear pore complex subsequent to docking. These results provide genetic evidence that Hsp70 acts during both targeting and translocation phases of nuclear transport, possibly as a molecular chaperone to promote the formation and stability of the Srp1p-NLS-cargo complex." @default.
• W2132320427 created "2016-06-24" @default.
• W2132320427 creator A5000470900 @default.
• W2132320427 creator A5002963865 @default.
• W2132320427 creator A5006779777 @default.
• W2132320427 creator A5021159543 @default.
• W2132320427 creator A5044811616 @default.
• W2132320427 creator A5072834548 @default.
• W2132320427 creator A5088302147 @default.
• W2132320427 date "1996-10-15" @default.
• W2132320427 modified "2023-09-26" @default.
• W2132320427 title "In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation." @default.
• W2132320427 cites W1482247598 @default.
• W2132320427 cites W1497947649 @default.
• W2132320427 cites W1599259441 @default.
• W2132320427 cites W1856840144 @default.
• W2132320427 cites W1902070349 @default.
• W2132320427 cites W1903209843 @default.
• W2132320427 cites W1939013880 @default.
• W2132320427 cites W1966767622 @default.
• W2132320427 cites W1970711633 @default.
• W2132320427 cites W1971227936 @default.
• W2132320427 cites W1978372269 @default.
• W2132320427 cites W1979394236 @default.
• W2132320427 cites W1989650768 @default.
• W2132320427 cites W2007121427 @default.
• W2132320427 cites W2007168999 @default.
• W2132320427 cites W2008267067 @default.
• W2132320427 cites W2012339382 @default.
• W2132320427 cites W2014293907 @default.
• W2132320427 cites W2015617558 @default.
• W2132320427 cites W2019894624 @default.
• W2132320427 cites W2022349106 @default.
• W2132320427 cites W2029306713 @default.
• W2132320427 cites W2029912871 @default.
• W2132320427 cites W2032515211 @default.
• W2132320427 cites W2034079946 @default.
• W2132320427 cites W2035091883 @default.
• W2132320427 cites W2036429346 @default.
• W2132320427 cites W2036742051 @default.
• W2132320427 cites W2043295775 @default.
• W2132320427 cites W2052881693 @default.
• W2132320427 cites W2054370975 @default.
• W2132320427 cites W2057493866 @default.
• W2132320427 cites W2058254034 @default.
• W2132320427 cites W2058477978 @default.
• W2132320427 cites W2060403740 @default.
• W2132320427 cites W2063055503 @default.
• W2132320427 cites W2063499197 @default.
• W2132320427 cites W2065235814 @default.
• W2132320427 cites W2065522041 @default.
• W2132320427 cites W2070662314 @default.
• W2132320427 cites W2070831615 @default.
• W2132320427 cites W2072523212 @default.
• W2132320427 cites W2075079336 @default.
• W2132320427 cites W2075450053 @default.
• W2132320427 cites W2081103615 @default.
• W2132320427 cites W2086725260 @default.
• W2132320427 cites W2087302056 @default.
• W2132320427 cites W2091285777 @default.
• W2132320427 cites W2093835976 @default.
• W2132320427 cites W2094745805 @default.
• W2132320427 cites W2098620367 @default.
• W2132320427 cites W2112659911 @default.
• W2132320427 cites W2117468486 @default.
• W2132320427 cites W2117954424 @default.
• W2132320427 cites W2120226363 @default.
• W2132320427 cites W2125778361 @default.
• W2132320427 cites W2129358952 @default.
• W2132320427 cites W2130500272 @default.
• W2132320427 cites W2146018121 @default.
• W2132320427 cites W2148913383 @default.
• W2132320427 cites W2150687522 @default.
• W2132320427 cites W2150857496 @default.
• W2132320427 cites W2157633445 @default.
• W2132320427 cites W2164931904 @default.
• W2132320427 cites W2165562991 @default.
• W2132320427 cites W2180706389 @default.
• W2132320427 cites W218971763 @default.
• W2132320427 cites W2252877470 @default.
• W2132320427 cites W4238100084 @default.
• W2132320427 cites W4240065971 @default.
• W2132320427 doi "https://doi.org/10.1083/jcb.135.2.329" @default.
• W2132320427 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2121037" @default.
• W2132320427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8896592" @default.
• W2132320427 hasPublicationYear "1996" @default.
• W2132320427 type Work @default.
• W2132320427 sameAs 2132320427 @default.
• W2132320427 citedByCount "202" @default.
• W2132320427 countsByYear W21323204272012 @default.
• W2132320427 countsByYear W21323204272013 @default.
• W2132320427 countsByYear W21323204272014 @default.
• W2132320427 countsByYear W21323204272015 @default.
• W2132320427 countsByYear W21323204272016 @default.
• W2132320427 countsByYear W21323204272018 @default.
• W2132320427 countsByYear W21323204272019 @default.
• W2132320427 countsByYear W21323204272020 @default.
• W2132320427 countsByYear W21323204272021 @default.

• next