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• W2132321829 abstract "The antigen-specific primary activation of CD4+ T cells was studied in vivo by adoptive transfer of ovalbumin-specific transgenic T cells (KJ1-26+ CD4+) following intranasal immunization with recombinant Streptococcus gordonii. A strain of S. gordonii expressing on its surface a model vaccine antigen fused to the ovalbumin (OVA) peptide from position 323 to 339 was constructed and used to study the OVA-specific T-cell activation in nasal mucosa-associated lymphoid tissue (NALT), lymph nodes, and spleens of mice immunized by the intranasal route. The recombinant strain, but not the wild type, activated the OVA-specific CD4+ T-cell population in the NALT (89% of KJ1-26+ CD4+ T cells) just 3 days following immunization. In the cervical lymph nodes and in the spleen, the percentage of proliferating cells was initially low, but it reached the peak of activation at day 5 (90%). This antigen-specific clonal expansion of KJ1-26+ CD4+ T cells after intranasal immunization was obtained with live and inactivated recombinant bacteria, and it indicates that the NALT is the site of antigen-specific T-cell priming." @default.
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• W2132321829 date "2006-05-01" @default.
• W2132321829 modified "2023-10-14" @default.
• W2132321829 title "In Vivo Activation of Naive CD4⁺T Cells in Nasal Mucosa-Associated Lymphoid Tissue following Intranasal Immunization with Recombinant<i>Streptococcus gordonii</i>" @default.
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• W2132321829 doi "https://doi.org/10.1128/iai.74.5.2760-2766.2006" @default.
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