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- W2132415886 abstract "Fluorescein diacetate (FDA), a fluorogenic vital stain, was used in conjunction with a silicon-intensified target (SIT) camera and computer-based image analysis to estimate the viability of cells of the inner cell mass (ICM) and trophectoderm (TE) of freshly collected and frozen-thawed horse embryos. Fluorescence intensities were greater (p<0.02) for fresh ICM (0.1500) and TE (0.1686) than for frozen-thawed ICM (0.0323) and TE (0.0694). Fluorescence intensity ratios (ICM:TE) also differed (p<0.004) between fresh (0.966) and frozen-thawed (0.482) embryos. These results indicate that 1) the use of FDA coupled with SIT camera image analysis is a repeatable, objective method for assessing embryo viability and 2) the freeze-thaw process is more detrimental to ICM cells than to TE cells of horse embryos." @default.
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- W2132415886 date "2010-06-10" @default.
- W2132415886 modified "2023-09-26" @default.
- W2132415886 title "Viability of inner cell mass versus trophectodermal cells of frozen-thawed horse embryos" @default.
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- W2132415886 doi "https://doi.org/10.1111/j.2042-3306.1989.tb04685.x" @default.
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