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• W2132457790 abstract "Abstract: [3H]Acetylcholine efflux and Na+-K+ ATPase ion pump activity were measured concomitantly in rat cortical synaptosomes. Ouabain (500 μM), strophanthidin (500 μM). and parachloromercuribenzene sulfonate (500 μM) each inhibited ouabain-sensitive 86Rb uptake and elevated [3H]acetylcholine release independently of the external calcium concentration. Veratridine (10 μM), electrical field stimulation (60 V, 60 Hz, 5-ms pulse duration), or the calcium ionophore A23187 (10μg/ml) also inhibited ouabain-sensitive 86Rb uptake and released [3H]acetylcholine, but via a calcium-dependent process. Veratridine-induced [3H]acetylcholine release and ion pump inhibition were correlated over a wide range of drug concentrations and both effects were blocked by pre-treatment with tetrodotoxin (1 μM). The rate of [3H]acetyl-choline efflux from superfused synaptosomes was increased within 15 s of exposure to ouabain, strophanthidin, veratridine, A23187, or field stimulation, while ouabain-sensitive 86Rb uptake was significantly decreased within a similar interval. These results suggest that [3H]acetylcholine release is due at least in part to inhibition of Na+-K+ ATPase." @default.
• W2132457790 created "2016-06-24" @default.
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• W2132457790 date "1981-02-01" @default.
• W2132457790 modified "2023-09-25" @default.
• W2132457790 title "Correlations Between Na⁺-K⁺ATPase Activity and Acetylcholine Release in Rat Cortical Synaptosomes" @default.
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• W2132457790 doi "https://doi.org/10.1111/j.1471-4159.1981.tb01616.x" @default.
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