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- W2132501554 abstract "Efflux pumps of the resistance nodulation division (RND) superfamily, such as AcrB, make a major contribution to multidrug resistance in Gram-negative bacteria. The development of inhibitors of the RND pumps would improve the efficacy of current and next-generation antibiotics. To date, however, only one inhibitor has been cocrystallized with AcrB. Thus, in silico structure-based analysis is essential for elucidating the interaction between other inhibitors and the efflux pumps. In this work, we used computer docking and molecular dynamics simulations to study the interaction between AcrB and the compound MBX2319, a novel pyranopyridine efflux pump inhibitor with potent activity against RND efflux pumps of Enterobacteriaceae species, as well as other known inhibitors (D13-9001, 1-[1-naphthylmethyl]-piperazine, and phenylalanylarginine-β-naphthylamide) and the binding of doxorubicin to the efflux-defective F610A variant of AcrB. We also analyzed the binding of a substrate, minocycline, for comparison. Our results show that MBX2319 binds very tightly to the lower part of the distal pocket in the B protomer of AcrB, strongly interacting with the phenylalanines lining the hydrophobic trap, where the hydrophobic portion of D13-9001 was found to bind by X-ray crystallography. Additionally, MBX2319 binds to AcrB in a manner that is similar to the way in which doxorubicin binds to the F610A variant of AcrB. In contrast, 1-(1-naphthylmethyl)-piperazine and phenylalanylarginine-β-naphthylamide appear to bind to somewhat different areas of the distal pocket in the B protomer of AcrB than does MBX2319. However, all inhibitors (except D13-9001) appear to distort the structure of the distal pocket, impairing the proper binding of substrates." @default.
- W2132501554 created "2016-06-24" @default.
- W2132501554 creator A5030274286 @default.
- W2132501554 creator A5057419582 @default.
- W2132501554 creator A5059598818 @default.
- W2132501554 creator A5086380170 @default.
- W2132501554 creator A5086764406 @default.
- W2132501554 date "2014-10-01" @default.
- W2132501554 modified "2023-10-18" @default.
- W2132501554 title "Molecular Mechanism of MBX2319 Inhibition of Escherichia coli AcrB Multidrug Efflux Pump and Comparison with Other Inhibitors" @default.
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- W2132501554 doi "https://doi.org/10.1128/aac.03283-14" @default.
- W2132501554 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4187987" @default.
- W2132501554 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25114133" @default.
- W2132501554 hasPublicationYear "2014" @default.
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