Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132568802> ?p ?o ?g. }
- W2132568802 endingPage "7082" @default.
- W2132568802 startingPage "7075" @default.
- W2132568802 abstract "ABSTRACT The genetic analysis of essential genes has been generally restricted to the use of conditional mutations, or inactivating chromosomal mutations, which require a complementing plasmid that must either be counterselected or lost to measure a phenotype. These approaches are limited because they do not permit the analysis of mutations suspected to affect a specific function of a protein, nor do they take advantage of the increasing abundance of structural and bioinformatics data for proteins. Using the dnaC gene as an example, we developed a genetic method that should permit the mutational analysis of other essential genes of Escherichia coli and related enterobacteria. The method consists of using a strain carrying a large deletion of the dnaC gene, which is complemented by a wild-type copy expressed from a plasmid that requires isopropyl-β- d -thiogalactopyranoside for maintenance. Under conditions in which this resident plasmid is lost, the method measures the function of a dnaC mutation encoded by a second plasmid. This methodology should be widely applicable to the genetic analysis of other essential genes." @default.
- W2132568802 created "2016-06-24" @default.
- W2132568802 creator A5016708001 @default.
- W2132568802 creator A5043350557 @default.
- W2132568802 creator A5061614134 @default.
- W2132568802 creator A5087916879 @default.
- W2132568802 date "2007-11-01" @default.
- W2132568802 modified "2023-09-26" @default.
- W2132568802 title "Genetic Method To Analyze Essential Genes of <i>Escherichia coli</i>" @default.
- W2132568802 cites W1493292322 @default.
- W2132568802 cites W1570361288 @default.
- W2132568802 cites W1591715670 @default.
- W2132568802 cites W1839052585 @default.
- W2132568802 cites W1923290408 @default.
- W2132568802 cites W1970341643 @default.
- W2132568802 cites W1970881542 @default.
- W2132568802 cites W1970928098 @default.
- W2132568802 cites W1976018721 @default.
- W2132568802 cites W1979784608 @default.
- W2132568802 cites W1982011873 @default.
- W2132568802 cites W1995227830 @default.
- W2132568802 cites W2026007247 @default.
- W2132568802 cites W2028941521 @default.
- W2132568802 cites W2039316348 @default.
- W2132568802 cites W2040409577 @default.
- W2132568802 cites W2050325858 @default.
- W2132568802 cites W2059265460 @default.
- W2132568802 cites W2060255621 @default.
- W2132568802 cites W2066179156 @default.
- W2132568802 cites W2076344000 @default.
- W2132568802 cites W2086204665 @default.
- W2132568802 cites W2088658210 @default.
- W2132568802 cites W2111241008 @default.
- W2132568802 cites W2116137883 @default.
- W2132568802 cites W2121431496 @default.
- W2132568802 cites W2133041897 @default.
- W2132568802 cites W2136670217 @default.
- W2132568802 cites W2141653906 @default.
- W2132568802 cites W2145674223 @default.
- W2132568802 cites W22874528 @default.
- W2132568802 doi "https://doi.org/10.1128/aem.01756-07" @default.
- W2132568802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2074942" @default.
- W2132568802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17873068" @default.
- W2132568802 hasPublicationYear "2007" @default.
- W2132568802 type Work @default.
- W2132568802 sameAs 2132568802 @default.
- W2132568802 citedByCount "15" @default.
- W2132568802 countsByYear W21325688022014 @default.
- W2132568802 countsByYear W21325688022016 @default.
- W2132568802 countsByYear W21325688022018 @default.
- W2132568802 countsByYear W21325688022020 @default.
- W2132568802 countsByYear W21325688022021 @default.
- W2132568802 crossrefType "journal-article" @default.
- W2132568802 hasAuthorship W2132568802A5016708001 @default.
- W2132568802 hasAuthorship W2132568802A5043350557 @default.
- W2132568802 hasAuthorship W2132568802A5061614134 @default.
- W2132568802 hasAuthorship W2132568802A5087916879 @default.
- W2132568802 hasBestOaLocation W21325688022 @default.
- W2132568802 hasConcept C104317684 @default.
- W2132568802 hasConcept C127716648 @default.
- W2132568802 hasConcept C14036430 @default.
- W2132568802 hasConcept C22744801 @default.
- W2132568802 hasConcept C23085057 @default.
- W2132568802 hasConcept C501734568 @default.
- W2132568802 hasConcept C54355233 @default.
- W2132568802 hasConcept C547475151 @default.
- W2132568802 hasConcept C70721500 @default.
- W2132568802 hasConcept C86803240 @default.
- W2132568802 hasConceptScore W2132568802C104317684 @default.
- W2132568802 hasConceptScore W2132568802C127716648 @default.
- W2132568802 hasConceptScore W2132568802C14036430 @default.
- W2132568802 hasConceptScore W2132568802C22744801 @default.
- W2132568802 hasConceptScore W2132568802C23085057 @default.
- W2132568802 hasConceptScore W2132568802C501734568 @default.
- W2132568802 hasConceptScore W2132568802C54355233 @default.
- W2132568802 hasConceptScore W2132568802C547475151 @default.
- W2132568802 hasConceptScore W2132568802C70721500 @default.
- W2132568802 hasConceptScore W2132568802C86803240 @default.
- W2132568802 hasIssue "21" @default.
- W2132568802 hasLocation W21325688021 @default.
- W2132568802 hasLocation W21325688022 @default.
- W2132568802 hasLocation W21325688023 @default.
- W2132568802 hasLocation W21325688024 @default.
- W2132568802 hasOpenAccess W2132568802 @default.
- W2132568802 hasPrimaryLocation W21325688021 @default.
- W2132568802 hasRelatedWork W1571771606 @default.
- W2132568802 hasRelatedWork W1873683136 @default.
- W2132568802 hasRelatedWork W1968976175 @default.
- W2132568802 hasRelatedWork W2038153601 @default.
- W2132568802 hasRelatedWork W2094445906 @default.
- W2132568802 hasRelatedWork W2143066563 @default.
- W2132568802 hasRelatedWork W2350652591 @default.
- W2132568802 hasRelatedWork W2414030161 @default.
- W2132568802 hasRelatedWork W2524850911 @default.
- W2132568802 hasRelatedWork W2594927926 @default.
- W2132568802 hasVolume "73" @default.
- W2132568802 isParatext "false" @default.
- W2132568802 isRetracted "false" @default.