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- W2132580407 abstract "Myostatin is a negative regulatory protein of growth and muscular development in several vertebrate species. However, for molluscs there is currently little information about the structure and function of the myostatin gene (MSTN), and its implications at the physiological and ecological levels. The aims of this study were to molecularly characterize the MSTN gene in Argopecten purpuratus (ApMSTN) and analyse gene expression patterns between wild and hatchery-bred populations. A complete ApMSTN sequence of 1,062 base pairs (bp) was obtained, which encodes a 354 amino acid. The amino acid sequence indicated a characteristic proteolytic processing site of MSTN (RXXR), nine conserved cysteine residues, and 344 and 919 bp corresponding to 5 0 UTR and 3 0 UTR, respectively. Furthermore, ApMSTN sequences revealed a total of four single-nucleotide polymorphisms (SNPs). Of these, SNP 2186G/A was only found in the wild population, while SNP 2305A/T was found in both populations. SNP þ779A/G, located in the coding region, was identified in wild scallops. In contrast, mutations identified in the 3 0 UTR were only found in hatchery-bred individuals. An upregulated expression pattern of ApMSTN was mainly associated with the adductor muscle of hatchery-bred scallops. The molecular differences found for the ApMSTN gene in A. purpuratus provide relevant information for future evaluations on the ecological implications of this gene." @default.
- W2132580407 created "2016-06-24" @default.
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- W2132580407 date "2014-03-25" @default.
- W2132580407 modified "2023-09-24" @default.
- W2132580407 title "The Myostatin gene of Argopecten purpuratus (ApMSTN): transcript expression and single-nucleotide polymorphism differences between wild and hatchery-bred populations" @default.
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- W2132580407 doi "https://doi.org/10.1093/mollus/eyu005" @default.
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