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• W2132620894 endingPage "1590" @default.
• W2132620894 startingPage "1587" @default.
• W2132620894 abstract "The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regulated C. albicans PHR1 and PHR2 genes in C. dubliniensis. Restriction fragment length polymorphism analysis suggests that there are significant sequence differences between the genes of the two species. In order to exploit this apparent difference, oligonucleotide primers based on the coding sequence of the C. albicans PHR1 structural gene were designed and used in PCR experiments. Use of these primers with C. albicans template DNA from 17 strains yielded a predicted 1.6-kb product, while C. dubliniensis template DNA from 19 strains yielded no product. We therefore propose that PCR using these primers is a rapid and reliable means of distinguishing the two germ tube- and chlamydospore-producing species C. albicans and C. dubliniensis." @default.
• W2132620894 created "2016-06-24" @default.
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• W2132620894 date "1999-05-01" @default.
• W2132620894 modified "2023-10-08" @default.
• W2132620894 title "Rapid PCR Test for Discriminating between <i>Candida albicans</i> and <i>Candida dubliniensis</i> Isolates Using Primers Derived from the pH-Regulated <i>PHR1</i> and <i>PHR2</i> Genes of <i>C. albicans</i>" @default.
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• W2132620894 doi "https://doi.org/10.1128/jcm.37.5.1587-1590.1999" @default.
• W2132620894 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/84840" @default.
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