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- W2132661442 abstract "Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation (µIP) method for the on-chip purification of proteins from complex mixtures is described. A 4 µl PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the µIP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised µIP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate." @default.
- W2132661442 created "2016-06-24" @default.
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- W2132661442 date "2010-01-01" @default.
- W2132661442 modified "2023-10-16" @default.
- W2132661442 title "On-chip immunoprecipitation for protein purification" @default.
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- W2132661442 doi "https://doi.org/10.1039/c005295g" @default.
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