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• W2132846390 abstract "Abstract The immune response and morphological changes in the gills of rainbow trout fry after immersion in hydrogen peroxide ( H 2 O 2 ), F lavobacterium psychrophilum or combined exposure were examined. The gills were sampled 4, 48, 125 and 192 h after exposure, and the regulation of expression of the following genes was investigated using q PCR : I g T , I g M , CD 8, CD 4, MHC I, MHC II, IL ‐4/13A, T c R ‐β, IL ‐10, IL ‐1β, IL ‐17, SAA and F ox P 3. Bacteria were not observed in haematoxylin‐and‐eosin‐stained gill tissue, but the presence of F . psychrophilum 16 S r RNA was detected using q PCR . The 16 S r RNA levels were correlated with gene expression. Although pretreatment with H 2 O 2 before immersion in F . psychrophilum did not significantly alter the amount of bacteria found in the gill, the immune response was influenced: exposure to F . psychrophilum resulted in a negative correlation with expression of IL ‐17c1, MHC I and MHC II, while pretreatment with H 2 O 2 resulted in a positive correlation with IL ‐4/13 A and I g M . Exposure to either H 2 O 2 or F . psychrophilum influenced the regulation of gene expression and damaged tissue. Exposure to both combined altered the immune response to infection and postponed healing of gill tissue." @default.
• W2132846390 created "2016-06-24" @default.
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• W2132846390 date "2014-02-18" @default.
• W2132846390 modified "2023-10-03" @default.
• W2132846390 title "Effect of hydrogen peroxide and/or <i>Flavobacterium psychrophilum</i> on the gills of rainbow trout, <i>Oncorhynchus mykiss</i> (Walbaum)" @default.
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• W2132846390 doi "https://doi.org/10.1111/jfd.12232" @default.
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