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• W2132860818 endingPage "177" @default.
• W2132860818 startingPage "169" @default.
• W2132860818 abstract "Oral Diseases (2012) 18, 169–177 Objectives: To isolate the CD133+CD44+ cells from human tongue squamous cell carcinoma (TSCC) Tca8113 cell line and investigate biological characteristics of them. Materials and methods: Immunomagnetic microbeads were applied to sort the CD133+CD44+ cells. Flow cytometry was used to detect isolation purity. The proliferation, clone-formation efficiencies, invasion and migration, gene expressions, and tumor-formation abilities were analyzed among CD133+CD44+, CD133−CD44−, and total population of cells. Results: The average purities of CD133+ and CD44+ cells reached 97.3% and 98.7%, respectively. The proliferation of CD133+CD44+ cells was significantly higher than the other two groups. The clone-forming efficiency of three groups was 70%, 8%, and 14%, respectively. The average invaded and migrated cell numbers of CD133+CD44+ and total population cells were 132 and 36.2, 311.6, and 156.2, respectively. The expressions of Bcl-2 and Sox2 in CD133+CD44+ cells were significantly higher than those in total population cells. A total of 104 CD133+CD44+ cells could form secondary tumors in nude mice, while the total population group needed 106 cells. Conclusions: The CD133+CD44+ subpopulation cells possess stem-like characteristics. They appear to be the potential targets for future biology therapy of human TSCC." @default.
• W2132860818 created "2016-06-24" @default.
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• W2132860818 date "2011-10-24" @default.
• W2132860818 modified "2023-10-18" @default.
• W2132860818 title "Biological characteristics of a cell subpopulation in tongue squamous cell carcinoma" @default.
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• W2132860818 doi "https://doi.org/10.1111/j.1601-0825.2011.01860.x" @default.
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