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- W2132881036 abstract "Nuclei were isolated from protoplasts obtained from Parthenocissus tricuspidata Crown Gall callus tissues. The effect of various isolation procedures, detergent or ultrasonication, on yield and quality of nuclei was studied. A standard procedure, based on the use of 5 × 10−3% Triton × 100 — 6% PVP — 20% glycerol, may be carried out in 30 min and gives 80 to 90% yield of nuclei in which RNA polymerase activity is retained." @default.
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- W2132881036 date "1978-07-01" @default.
- W2132881036 modified "2023-10-01" @default.
- W2132881036 title "High Yield Isolation of Nuclei from Plant Protoplasts" @default.
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- W2132881036 doi "https://doi.org/10.1111/j.1399-3054.1978.tb02564.x" @default.
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