FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2132922134> ?p ?o ?g. }

• W2132922134 endingPage "3453" @default.
• W2132922134 startingPage "3446" @default.
• W2132922134 abstract "We report here the construction, characterization, and application of a bacterial bioreporter for fructose and sucrose that was designed to monitor the availability of these sugars to microbial colonizers of the phyllosphere. Plasmid pP(fruB)-gfp[AAV] carries the Escherichia coli fruB promoter upstream from the gfp[AAV] allele that codes for an unstable variant of green fluorescent protein (GFP). In Erwinia herbicola, this plasmid brings about the accumulation of GFP fluorescence in response to both fructose and sucrose. Cells of E. herbicola (pP(fruB)-gfp[AAV]) were sprayed onto bean plants, recovered from leaves at various time intervals after inoculation, and analyzed individually for GFP content by quantitative analysis of digital microscope images. We observed a positive correlation between single-cell GFP accumulation and ribosomal content as determined by fluorescence in situ hybridization, indicating that foliar growth of E. herbicola occurred at the expense of fructose and/or sucrose. One hour after inoculation, nearly all bioreporter cells appeared to be actively engaged in fructose consumption. This fraction dropped to approximately 11% after 7 h and to approximately 1% a day after inoculation. This pattern suggests a highly heterogeneous availability of fructose to individual E. herbicola cells as they colonize the phyllosphere. We estimated that individual cells were exposed to local initial fructose abundances ranging from less than 0.15 pg fructose to more than 4.6 pg." @default.
• W2132922134 created "2016-06-24" @default.
• W2132922134 creator A5028713092 @default.
• W2132922134 creator A5082220656 @default.
• W2132922134 date "2001-03-13" @default.
• W2132922134 modified "2023-10-18" @default.
• W2132922134 title "Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere" @default.
• W2132922134 cites W1489942189 @default.
• W2132922134 cites W1540505601 @default.
• W2132922134 cites W1550243199 @default.
• W2132922134 cites W1556809021 @default.
• W2132922134 cites W1595974508 @default.
• W2132922134 cites W173805554 @default.
• W2132922134 cites W1780529601 @default.
• W2132922134 cites W1914117083 @default.
• W2132922134 cites W1931147290 @default.
• W2132922134 cites W1931709758 @default.
• W2132922134 cites W1977994313 @default.
• W2132922134 cites W1980308497 @default.
• W2132922134 cites W1984808670 @default.
• W2132922134 cites W199950176 @default.
• W2132922134 cites W2018430937 @default.
• W2132922134 cites W2019457185 @default.
• W2132922134 cites W2021070765 @default.
• W2132922134 cites W2023593720 @default.
• W2132922134 cites W2025160765 @default.
• W2132922134 cites W2038189090 @default.
• W2132922134 cites W2042519751 @default.
• W2132922134 cites W2056097036 @default.
• W2132922134 cites W2056569560 @default.
• W2132922134 cites W2062221023 @default.
• W2132922134 cites W2071105399 @default.
• W2132922134 cites W2072389303 @default.
• W2132922134 cites W2074063804 @default.
• W2132922134 cites W2074600137 @default.
• W2132922134 cites W2094330479 @default.
• W2132922134 cites W2103260421 @default.
• W2132922134 cites W2103278057 @default.
• W2132922134 cites W2106184123 @default.
• W2132922134 cites W2113524153 @default.
• W2132922134 cites W2126489244 @default.
• W2132922134 cites W2127068865 @default.
• W2132922134 cites W2159916252 @default.
• W2132922134 cites W2160164321 @default.
• W2132922134 cites W2168810410 @default.
• W2132922134 cites W2169628982 @default.
• W2132922134 cites W2170042470 @default.
• W2132922134 cites W2172007347 @default.
• W2132922134 cites W2174222776 @default.
• W2132922134 cites W2317645507 @default.
• W2132922134 cites W4230423915 @default.
• W2132922134 cites W98183580 @default.
• W2132922134 doi "https://doi.org/10.1073/pnas.061629598" @default.
• W2132922134 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/30673" @default.
• W2132922134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11248098" @default.
• W2132922134 hasPublicationYear "2001" @default.
• W2132922134 type Work @default.
• W2132922134 sameAs 2132922134 @default.
• W2132922134 citedByCount "314" @default.
• W2132922134 countsByYear W21329221342012 @default.
• W2132922134 countsByYear W21329221342013 @default.
• W2132922134 countsByYear W21329221342014 @default.
• W2132922134 countsByYear W21329221342015 @default.
• W2132922134 countsByYear W21329221342016 @default.
• W2132922134 countsByYear W21329221342017 @default.
• W2132922134 countsByYear W21329221342018 @default.
• W2132922134 countsByYear W21329221342019 @default.
• W2132922134 countsByYear W21329221342020 @default.
• W2132922134 countsByYear W21329221342021 @default.
• W2132922134 countsByYear W21329221342022 @default.
• W2132922134 countsByYear W21329221342023 @default.
• W2132922134 crossrefType "journal-article" @default.
• W2132922134 hasAuthorship W2132922134A5028713092 @default.
• W2132922134 hasAuthorship W2132922134A5082220656 @default.
• W2132922134 hasBestOaLocation W21329221341 @default.
• W2132922134 hasConcept C104317684 @default.
• W2132922134 hasConcept C142613039 @default.
• W2132922134 hasConcept C144027150 @default.
• W2132922134 hasConcept C180032290 @default.
• W2132922134 hasConcept C205626393 @default.
• W2132922134 hasConcept C2776970464 @default.
• W2132922134 hasConcept C2777108408 @default.
• W2132922134 hasConcept C2778597767 @default.
• W2132922134 hasConcept C31903555 @default.
• W2132922134 hasConcept C523546767 @default.
• W2132922134 hasConcept C54355233 @default.
• W2132922134 hasConcept C547475151 @default.
• W2132922134 hasConcept C55493867 @default.
• W2132922134 hasConcept C86803240 @default.
• W2132922134 hasConcept C89423630 @default.
• W2132922134 hasConceptScore W2132922134C104317684 @default.
• W2132922134 hasConceptScore W2132922134C142613039 @default.
• W2132922134 hasConceptScore W2132922134C144027150 @default.
• W2132922134 hasConceptScore W2132922134C180032290 @default.
• W2132922134 hasConceptScore W2132922134C205626393 @default.
• W2132922134 hasConceptScore W2132922134C2776970464 @default.
• W2132922134 hasConceptScore W2132922134C2777108408 @default.
• W2132922134 hasConceptScore W2132922134C2778597767 @default.

• next