Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133080233> ?p ?o ?g. }
- W2133080233 endingPage "247" @default.
- W2133080233 startingPage "238" @default.
- W2133080233 abstract "Reactive oxygen species (ROS) are produced by dendritic cells (DCs) during antigen presentation in contact hypersensitivity (CHS). ROS cause a number of non-enzymatic protein modifications, such as carbonylation. Carbonylated proteins in DCs in response to hapten have not been fully identified yet. To identify the proteins carbonylated by ROS, murine epidermis-derived DC line XS106 was challenged with a hapten, 2,4,6-trinitrobenzene sulphonic acid (TNBS). MALDI-TOF analysis revealed that heat-shock protein 70 (HSP70) was one of the carbonylated proteins induced by TNBS. To verify the role of HSP70 in TNBS-treated XS106 cell, we fused protein transduction domain (PTD) with HSP70 to facilitate protein delivery into the cell. The transfected fusion protein HSP70 within the cell caused transient increase of the cellular level of HSP70. Transient increase of HSP70 level in XS-106 DCs resulted in inhibition of ROS production, carbonylation of HSP70, p38 MAPK activation and subsequently IL-12 secretion. To investigate the effects of PTD-HSP70 in vivo, ear-swelling experiments with 2,4,6-trinitro-1-chlorobenzene (TNCB) were performed in BALB/c mice. Pretreatment of PTD-HSP70 reduced the CHS response to TNCB in vivo. We report here that carbonylation of HSP70 by ROS is associated with the pathogenesis of CHS, suggesting possibility of HSP70-targeting therapy in CHS." @default.
- W2133080233 created "2016-06-24" @default.
- W2133080233 creator A5000007655 @default.
- W2133080233 creator A5013708156 @default.
- W2133080233 creator A5024404157 @default.
- W2133080233 creator A5025367936 @default.
- W2133080233 creator A5030240308 @default.
- W2133080233 creator A5047075463 @default.
- W2133080233 creator A5054586839 @default.
- W2133080233 creator A5070738081 @default.
- W2133080233 creator A5071998677 @default.
- W2133080233 creator A5082575993 @default.
- W2133080233 creator A5088345588 @default.
- W2133080233 date "2013-08-22" @default.
- W2133080233 modified "2023-10-12" @default.
- W2133080233 title "The Antioxidative Effect of Heat-Shock Protein 70 in Dendritic Cells" @default.
- W2133080233 cites W125353669 @default.
- W2133080233 cites W1481246627 @default.
- W2133080233 cites W1507205945 @default.
- W2133080233 cites W1538733903 @default.
- W2133080233 cites W168224620 @default.
- W2133080233 cites W1866826175 @default.
- W2133080233 cites W1934356977 @default.
- W2133080233 cites W1939850337 @default.
- W2133080233 cites W1963971058 @default.
- W2133080233 cites W1964987933 @default.
- W2133080233 cites W1965909642 @default.
- W2133080233 cites W1971313680 @default.
- W2133080233 cites W1977178308 @default.
- W2133080233 cites W1977950302 @default.
- W2133080233 cites W1980547319 @default.
- W2133080233 cites W1983601075 @default.
- W2133080233 cites W1995486038 @default.
- W2133080233 cites W1996382471 @default.
- W2133080233 cites W1998411300 @default.
- W2133080233 cites W2004143904 @default.
- W2133080233 cites W2012461405 @default.
- W2133080233 cites W2019583554 @default.
- W2133080233 cites W2023746083 @default.
- W2133080233 cites W2036540780 @default.
- W2133080233 cites W2039535160 @default.
- W2133080233 cites W2040027128 @default.
- W2133080233 cites W2050789184 @default.
- W2133080233 cites W2051083202 @default.
- W2133080233 cites W2060630008 @default.
- W2133080233 cites W2080103294 @default.
- W2133080233 cites W2088335071 @default.
- W2133080233 cites W2088451184 @default.
- W2133080233 cites W2089984249 @default.
- W2133080233 cites W2091365069 @default.
- W2133080233 cites W2091767991 @default.
- W2133080233 cites W2092279282 @default.
- W2133080233 cites W2106162247 @default.
- W2133080233 cites W2110064348 @default.
- W2133080233 cites W2118954538 @default.
- W2133080233 cites W2121699992 @default.
- W2133080233 cites W2124271058 @default.
- W2133080233 cites W2125625534 @default.
- W2133080233 cites W2135418193 @default.
- W2133080233 cites W2141362920 @default.
- W2133080233 cites W2167187961 @default.
- W2133080233 cites W1584262534 @default.
- W2133080233 doi "https://doi.org/10.1111/sji.12078" @default.
- W2133080233 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23679814" @default.
- W2133080233 hasPublicationYear "2013" @default.
- W2133080233 type Work @default.
- W2133080233 sameAs 2133080233 @default.
- W2133080233 citedByCount "3" @default.
- W2133080233 countsByYear W21330802332015 @default.
- W2133080233 countsByYear W21330802332020 @default.
- W2133080233 crossrefType "journal-article" @default.
- W2133080233 hasAuthorship W2133080233A5000007655 @default.
- W2133080233 hasAuthorship W2133080233A5013708156 @default.
- W2133080233 hasAuthorship W2133080233A5024404157 @default.
- W2133080233 hasAuthorship W2133080233A5025367936 @default.
- W2133080233 hasAuthorship W2133080233A5030240308 @default.
- W2133080233 hasAuthorship W2133080233A5047075463 @default.
- W2133080233 hasAuthorship W2133080233A5054586839 @default.
- W2133080233 hasAuthorship W2133080233A5070738081 @default.
- W2133080233 hasAuthorship W2133080233A5071998677 @default.
- W2133080233 hasAuthorship W2133080233A5082575993 @default.
- W2133080233 hasAuthorship W2133080233A5088345588 @default.
- W2133080233 hasBestOaLocation W21330802331 @default.
- W2133080233 hasConcept C104317684 @default.
- W2133080233 hasConcept C150903083 @default.
- W2133080233 hasConcept C153911025 @default.
- W2133080233 hasConcept C185592680 @default.
- W2133080233 hasConcept C205260736 @default.
- W2133080233 hasConcept C207001950 @default.
- W2133080233 hasConcept C2776151105 @default.
- W2133080233 hasConcept C2780829032 @default.
- W2133080233 hasConcept C2911203004 @default.
- W2133080233 hasConcept C48349386 @default.
- W2133080233 hasConcept C55493867 @default.
- W2133080233 hasConcept C68991219 @default.
- W2133080233 hasConcept C86803240 @default.
- W2133080233 hasConcept C95444343 @default.
- W2133080233 hasConceptScore W2133080233C104317684 @default.