FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133175902> ?p ?o ?g. }

• W2133175902 endingPage "198" @default.
• W2133175902 startingPage "187" @default.
• W2133175902 abstract "Tripartite ATP-independent periplasmic (TRAP) transporters are relatively common prokaryotic secondary transporters which comprise an extracytoplasmic solute receptor (ESR) protein and two dissimilar membrane proteins or domains, yet the substrates and physiological functions of only a few of these systems are so far known. In this study, a biophysical approach was used to identify the ligands for the purified Rhodobacter capsulatus RRC01191 and Escherichia coli YiaO proteins, which are members of two phylogenetically distinct families of TRAP-ESRs found in diverse bacteria. In contrast to previous indirect evidence pointing to RRC01191 orthologues being involved in polyol uptake, it was shown that RRC01191 binds pyruvate, 2-oxobutyrate and a broad range of aliphatic monocarboxylic 2-oxoacid anions with varying affinities (K d values 0·08–3 μM), consistent with a predicted role in monocarboxylate transport related to branched-chain amino-acid biosynthesis. The E. coli YiaMNO TRAP transporter has previously been proposed to be an l-xylulose uptake system [ Plantinga et al. (2004) Mol Membr Biol 21, 51–57], but purified YiaO did not bind l- or d-xylulose as judged by fluorescence spectroscopy, circular dichroism or mass spectrometry. Instead, these techniques showed that a breakdown product of l-ascorbate, 2,3-diketo-l-gulonate (2,3-DKG), binds by a simple one-step mechanism with sub-micromolar affinity. The data provide the first evidence for the existence of ESR-dependent transporters for 2-oxoacids and 2,3-DKG, homologues of which appear to be widespread amongst prokaryotes. The results also underline the utility of direct ESR ligand-binding studies for TRAP transporter characterization" @default.
• W2133175902 created "2016-06-24" @default.
• W2133175902 creator A5028794362 @default.
• W2133175902 creator A5071488584 @default.
• W2133175902 creator A5080646179 @default.
• W2133175902 creator A5081218074 @default.
• W2133175902 creator A5090083154 @default.
• W2133175902 date "2006-01-01" @default.
• W2133175902 modified "2023-09-23" @default.
• W2133175902 title "Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters" @default.
• W2133175902 cites W1551627361 @default.
• W2133175902 cites W1557778578 @default.
• W2133175902 cites W1563805113 @default.
• W2133175902 cites W1566208906 @default.
• W2133175902 cites W1583331377 @default.
• W2133175902 cites W1594136944 @default.
• W2133175902 cites W1601184842 @default.
• W2133175902 cites W1608493062 @default.
• W2133175902 cites W1845106937 @default.
• W2133175902 cites W1898768752 @default.
• W2133175902 cites W1948289007 @default.
• W2133175902 cites W1964126053 @default.
• W2133175902 cites W1977189959 @default.
• W2133175902 cites W1978561525 @default.
• W2133175902 cites W1999080144 @default.
• W2133175902 cites W2006355699 @default.
• W2133175902 cites W2018127318 @default.
• W2133175902 cites W2038124483 @default.
• W2133175902 cites W2042580941 @default.
• W2133175902 cites W2045329580 @default.
• W2133175902 cites W2045799787 @default.
• W2133175902 cites W2048712121 @default.
• W2133175902 cites W2053129511 @default.
• W2133175902 cites W2055956723 @default.
• W2133175902 cites W2063580869 @default.
• W2133175902 cites W2067167446 @default.
• W2133175902 cites W2068288134 @default.
• W2133175902 cites W2084613580 @default.
• W2133175902 cites W2087750699 @default.
• W2133175902 cites W2091168091 @default.
• W2133175902 cites W2095292926 @default.
• W2133175902 cites W2097382368 @default.
• W2133175902 cites W2101093608 @default.
• W2133175902 cites W2103623969 @default.
• W2133175902 cites W2103630321 @default.
• W2133175902 cites W2105381452 @default.
• W2133175902 cites W2119452210 @default.
• W2133175902 cites W2121481633 @default.
• W2133175902 cites W2125347557 @default.
• W2133175902 cites W2126229871 @default.
• W2133175902 cites W2127126156 @default.
• W2133175902 cites W2129386299 @default.
• W2133175902 cites W2131359987 @default.
• W2133175902 cites W2133695192 @default.
• W2133175902 cites W2145267759 @default.
• W2133175902 cites W2147018428 @default.
• W2133175902 cites W2148913876 @default.
• W2133175902 cites W2149676844 @default.
• W2133175902 cites W2150354192 @default.
• W2133175902 cites W2161746138 @default.
• W2133175902 cites W2167048995 @default.
• W2133175902 cites W2169563465 @default.
• W2133175902 cites W4239748070 @default.
• W2133175902 cites W4244979914 @default.
• W2133175902 doi "https://doi.org/10.1099/mic.0.28334-0" @default.
• W2133175902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16385129" @default.
• W2133175902 hasPublicationYear "2006" @default.
• W2133175902 type Work @default.
• W2133175902 sameAs 2133175902 @default.
• W2133175902 citedByCount "48" @default.
• W2133175902 countsByYear W21331759022012 @default.
• W2133175902 countsByYear W21331759022013 @default.
• W2133175902 countsByYear W21331759022014 @default.
• W2133175902 countsByYear W21331759022015 @default.
• W2133175902 countsByYear W21331759022016 @default.
• W2133175902 countsByYear W21331759022017 @default.
• W2133175902 countsByYear W21331759022018 @default.
• W2133175902 countsByYear W21331759022019 @default.
• W2133175902 countsByYear W21331759022020 @default.
• W2133175902 countsByYear W21331759022021 @default.
• W2133175902 crossrefType "journal-article" @default.
• W2133175902 hasAuthorship W2133175902A5028794362 @default.
• W2133175902 hasAuthorship W2133175902A5071488584 @default.
• W2133175902 hasAuthorship W2133175902A5080646179 @default.
• W2133175902 hasAuthorship W2133175902A5081218074 @default.
• W2133175902 hasAuthorship W2133175902A5090083154 @default.
• W2133175902 hasBestOaLocation W21331759021 @default.
• W2133175902 hasConcept C104317684 @default.
• W2133175902 hasConcept C115335371 @default.
• W2133175902 hasConcept C143065580 @default.
• W2133175902 hasConcept C144647389 @default.
• W2133175902 hasConcept C146587185 @default.
• W2133175902 hasConcept C149011108 @default.
• W2133175902 hasConcept C185592680 @default.
• W2133175902 hasConcept C190114821 @default.
• W2133175902 hasConcept C201663137 @default.
• W2133175902 hasConcept C2777850835 @default.
• W2133175902 hasConcept C41625074 @default.

• next