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• W2133178247 abstract "Plant regeneration from immature embryos may follow different pathways. First, one may distinguish between a direct and an indirect way. The former implies the de novo development of meristems on the immature embryo from which new expiants originate. In the case of indirect regeneration, the development of new plantlets from the expiant is interrupted by an intervening callus phase. A second distinction in the regeneration process is made between organo-genesis, or shoot morphogenesis, and somatic embryogenesis. In the case of organogenesis, shoots are formed upon the expiant (directly) or on callus tissue (indirectly). Similarly, somatic embryos may develop directly or indirectly, depending on the absence or presence of an intervening callus phase. Figure 1 shows the different morphogenetic ways of regeneration as observed in embryo cultures of Zea mays (Green and Phillips 1975; Springer et al. 1979; Lu et al. 1982; Armstrong and Green 1985; Vasil et al. 1985; Fransz and Schel 1987). All studies used immature embryos with a scutellum length of 1.5-2 mm, which proved to be a sufficient specification of the required developmental stage. This chapter comprises a structural analysis of these various processes by light and electron microscopy." @default.
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• W2133178247 date "1994-01-01" @default.
• W2133178247 modified "2023-09-25" @default.
• W2133178247 title "Ultrastructural Studies on Callus Development and Somatic Embryogenesis in Zea mays L" @default.
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• W2133178247 doi "https://doi.org/10.1007/978-3-642-57968-4_4" @default.
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