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• W2133193898 abstract "Human atherosclerotic plaques have lipid compositions that fall in the three-phase region of the phase diagram of the major lipids of plaques, cholesterol, cholesterol ester, and phospholipid. We attempted to isolate the three lipid phases from both plaque homogenates and from extracted total plaque lipids using discontinuous sucrose density gradient centrifugation. The gradient was designed to isolate the cholesterol ester-rich phase (d 5 1.000 g/ml), the phospholipid-rich phase (d 5 1.028), the cholesterol monohydrate crystal phase (d < 1.054), and more dense nonlipid plaque constituents sedimenting at d = 1.054. The top layer of homogenized plaque (d < 1.00 g/ml) consisted of lipid droplets composed of 79.2% cholesterol ester, 7.8% triglyceride, 8.5% cholesterol, and 4.5% phospholipid. The top layer of extracted total plaque lipids had a similar composition. This composition approximates the cholesterol ester phase predicted by the phase diagram. The top layer of the extracted lipid system comprised 52% of total lipids closely approximating the 50% calculated from the phase diagram. However, only 20.4% of total plaque homogenate lipids were recovered in the top layer, indicating that some cholesterol ester was trapped or bound by other plaque components. The d = 1.028 layer revealed, in addition to the phospholipid phase, some cholesterol crystals, and in homogenate gradients, some lipid droplets. The composition of the homogenate d = 1.028 layer was consistent with the coexistence of all three lipid phases. The composition of the pure plaque lipid system d = 1.028 layer was consistent with the isolation of the phospholipid phase, but contaminated with some cholesterol crystals. The d = 1.054 layers from both extracted plaque lipids and plaque homogenate were rich in cholesterol monohydrate crystals. Homogenate layers were filtered and recentrifuged on a density gradient to further purify the crystals. The composition of the purified crystals was 88.0% cholesterol, 10.0% cholesterol ester, 1.1% triglyceride, and 0.9% phospholipid. The d = 1.054 layer of the extracted lipid system had a similar composition. Thus, two of the lipid phases of plaques, the cholesterol ester phase and the cholesterol crystalline phase, were isolated in relatively pure form. Almost all the" @default.
• W2133193898 created "2016-06-24" @default.
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• W2133193898 date "1980-10-01" @default.
• W2133193898 modified "2023-10-10" @default.
• W2133193898 title "Isolation and and partial characterization of the lipid phases of human atherosclerotic plaques." @default.
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• W2133193898 doi "https://doi.org/10.1016/s0021-9258(18)43457-6" @default.
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