Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133274316> ?p ?o ?g. }
- W2133274316 endingPage "1831" @default.
- W2133274316 startingPage "1823" @default.
- W2133274316 abstract "Background context Degeneration of the intervertebral disc is often associated with low back pain and increased infiltration of nerve fibers originating from dorsal root ganglia (DRG). The degenerated disc is also characterized by the presence of proinflammatory cytokines, which may influence axonal outgrowth. Toward an improved understanding of the growth of DRG neurons into compliant extracellular matrices, we developed a novel experimental system to measure axonal outgrowth of adult rat lumbar DRG neurons within three-dimensional (3D) collagen hydrogels and used this system to examine the effects of interleukin 1β (IL-1β) and tumor necrosis factor (TNF)-α treatment. Purpose The aim was to investigate the effects of proinflammatory cytokines on 3D neuronal growth into collagen matrices. Study design This was an in vitro study of neurite outgrowth from adult rat lumbar DRG into collagen gels in response to IL-1β and TNF-α. Methods Lumbar DRG were obtained from adult Sprague Dawley rats, bisected to expose cell bodies and placed onto collagen gel constructs prepared in 24-well Transwell inserts. Dorsal root ganglia were then treated with nerve growth factor (NGF)–free Neurobasal media (negative control) or NGF-supplemented media containing 0, 1, and 10 ng/mL of IL-1β and TNF-α. After 7 days, collagen gel–DRG constructs were immunostained for phosphorylated neurofilament, an axonal marker. Simple Neurite Tracer (Fiji/ImageJ) was used to quantify 3D axonal outgrowth from confocal image stacks. Data were analyzed using one-way analysis of variance, with Tukey HSD post hoc correction at a level of p<.05. Results Immunostaining showed robust axonal outgrowth into collagen gels from all NGF-treated DRG. The negative control demonstrated very few and short neurites. Tumor necrosis factor-α (1 and 10 ng/mL) significantly inhibited axonal outgrowth compared with NGF-only media (p<.026 and p<.02, respectively). After IL-1β treatment, average axon length was 10% lower at 1 ng/mL and 7.5% higher at 10 ng/mL, but these differences were not statistically significant. Among cytokine treatments, however, average axon length in the IL-1β (10 ng/mL) group was significantly higher than that in the other groups (p<.05). Conclusions A novel 3D collagen gel culture system was used to investigate factors modulating neuronal ingrowth. Our results showed that NGF was necessary to promote neurite growth into collagen gels. In the presence of proinflammatory cytokines, high concentrations of IL-1β induced significantly higher axonal outgrowth than TNF-α and low levels of IL-1β." @default.
- W2133274316 created "2016-06-24" @default.
- W2133274316 creator A5000891940 @default.
- W2133274316 creator A5024155695 @default.
- W2133274316 creator A5026893569 @default.
- W2133274316 creator A5075507156 @default.
- W2133274316 date "2015-08-01" @default.
- W2133274316 modified "2023-09-23" @default.
- W2133274316 title "Effects of proinflammatory cytokines on axonal outgrowth from adult rat lumbar dorsal root ganglia using a novel three-dimensional culture system" @default.
- W2133274316 cites W1453797714 @default.
- W2133274316 cites W1486651587 @default.
- W2133274316 cites W1936083277 @default.
- W2133274316 cites W1981489213 @default.
- W2133274316 cites W1982289658 @default.
- W2133274316 cites W1982728405 @default.
- W2133274316 cites W1998968944 @default.
- W2133274316 cites W2000654827 @default.
- W2133274316 cites W2003224769 @default.
- W2133274316 cites W2004131962 @default.
- W2133274316 cites W2006563794 @default.
- W2133274316 cites W2007353316 @default.
- W2133274316 cites W2012100020 @default.
- W2133274316 cites W2012808248 @default.
- W2133274316 cites W2015869069 @default.
- W2133274316 cites W2019911937 @default.
- W2133274316 cites W2019921106 @default.
- W2133274316 cites W2026074657 @default.
- W2133274316 cites W2029090192 @default.
- W2133274316 cites W2029345901 @default.
- W2133274316 cites W2031608440 @default.
- W2133274316 cites W2034395889 @default.
- W2133274316 cites W2038456030 @default.
- W2133274316 cites W2039948531 @default.
- W2133274316 cites W2041731091 @default.
- W2133274316 cites W2044474568 @default.
- W2133274316 cites W2058428380 @default.
- W2133274316 cites W2062203127 @default.
- W2133274316 cites W2064613397 @default.
- W2133274316 cites W2066429128 @default.
- W2133274316 cites W2070353807 @default.
- W2133274316 cites W2072158777 @default.
- W2133274316 cites W2081739880 @default.
- W2133274316 cites W2081919740 @default.
- W2133274316 cites W2097530133 @default.
- W2133274316 cites W2104827963 @default.
- W2133274316 cites W2115577615 @default.
- W2133274316 cites W2117724039 @default.
- W2133274316 cites W2121787709 @default.
- W2133274316 cites W2133809313 @default.
- W2133274316 cites W2134318006 @default.
- W2133274316 cites W2143348633 @default.
- W2133274316 cites W2148769724 @default.
- W2133274316 cites W2150115576 @default.
- W2133274316 cites W2150271931 @default.
- W2133274316 cites W2154731315 @default.
- W2133274316 cites W2156011701 @default.
- W2133274316 cites W2162628709 @default.
- W2133274316 cites W2163184722 @default.
- W2133274316 cites W2163190968 @default.
- W2133274316 cites W2280235071 @default.
- W2133274316 cites W2328082294 @default.
- W2133274316 doi "https://doi.org/10.1016/j.spinee.2015.03.017" @default.
- W2133274316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25797812" @default.
- W2133274316 hasPublicationYear "2015" @default.
- W2133274316 type Work @default.
- W2133274316 sameAs 2133274316 @default.
- W2133274316 citedByCount "12" @default.
- W2133274316 countsByYear W21332743162016 @default.
- W2133274316 countsByYear W21332743162017 @default.
- W2133274316 countsByYear W21332743162018 @default.
- W2133274316 countsByYear W21332743162019 @default.
- W2133274316 countsByYear W21332743162020 @default.
- W2133274316 countsByYear W21332743162021 @default.
- W2133274316 countsByYear W21332743162022 @default.
- W2133274316 crossrefType "journal-article" @default.
- W2133274316 hasAuthorship W2133274316A5000891940 @default.
- W2133274316 hasAuthorship W2133274316A5024155695 @default.
- W2133274316 hasAuthorship W2133274316A5026893569 @default.
- W2133274316 hasAuthorship W2133274316A5075507156 @default.
- W2133274316 hasConcept C105702510 @default.
- W2133274316 hasConcept C113246987 @default.
- W2133274316 hasConcept C126322002 @default.
- W2133274316 hasConcept C140530291 @default.
- W2133274316 hasConcept C142724271 @default.
- W2133274316 hasConcept C151730666 @default.
- W2133274316 hasConcept C157207234 @default.
- W2133274316 hasConcept C164027704 @default.
- W2133274316 hasConcept C170493617 @default.
- W2133274316 hasConcept C17991360 @default.
- W2133274316 hasConcept C185592680 @default.
- W2133274316 hasConcept C202751555 @default.
- W2133274316 hasConcept C203014093 @default.
- W2133274316 hasConcept C204232928 @default.
- W2133274316 hasConcept C2776281502 @default.
- W2133274316 hasConcept C2776914184 @default.
- W2133274316 hasConcept C2778423431 @default.
- W2133274316 hasConcept C2779343474 @default.
- W2133274316 hasConcept C4224716 @default.