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• W2133293208 startingPage "1933" @default.
• W2133293208 abstract "A procedure for the construction of 3′-end labelled yeast tRNA harboring substitutions or additions of any desired nucleotide 1n T-steni and T-loop (position 57 to 61) has been developed. This was done by in vitro enzymatic manipulations of the yeast tRNA p Involving specific “fiydroly-s1s with RNases, phosphorylation and dephosphorylation with T4 polynucleotide kinase and 11 gat ion with T4 RNA Ugase. Using this procedure we have replaced conserved or semi-conserved nucleotides located 1n position 57 tp 61 of yeast tRNA Asp . He have also constructed different yeast tRNA 515 with eight bases Instead of seven 1n T-loop. Further use of these tRNA Asp variants will be discussed with the help of the crystallographic three-dimensional structure" @default.
• W2133293208 created "2016-06-24" @default.
• W2133293208 creator A5044061478 @default.
• W2133293208 creator A5049411069 @default.
• W2133293208 date "1987-01-01" @default.
• W2133293208 modified "2023-09-24" @default.
• W2133293208 title "<i>in vitro</i>construction of yeast tRNA^Aspvariants: nucleotide substitutions and additions in T-stem and T-loop" @default.
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• W2133293208 doi "https://doi.org/10.1093/nar/15.5.1933" @default.
• W2133293208 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/340609" @default.
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