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- W2133311302 abstract "A series of 4,7-π-extended 2,1,3-benzothiadiazoles (BTDs) with different molecular architectures, in particular, the organic dyes based on the 4-(arylethynyl)-7-(4-methoxy)-2,1,3-benzothiazole skeleton, can be used at very low concentrations (down to 10 μM) to detect DNA at 1 ppm in phosphate buffer solutions. Upon binding to DNA, these dyes showed an exponential increase in the fluorescence intensity (hyperchromic effect) and a red shift (1–5 nm) in the long-wavelength emission maxima. Pre-steady state kinetic experiments (stopped-flow) demonstrated the fast dye interaction with the biomacromolecules of DNA with an increase in fluorescence, especially with non-symmetrical BTDs containing an ethynyl spacer. An intercalation model could be proposed based on the photophysical properties, X-ray analysis, and theoretical calculations (ab initio). In this model, the intercalation occurs on the ethynyl side of the BTD, and as a consequence, the PhOMe portion is free to perform the ICT process with the BTD core and stabilizes it in the excited state." @default.
- W2133311302 created "2016-06-24" @default.
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- W2133311302 date "2010-01-01" @default.
- W2133311302 modified "2023-10-13" @default.
- W2133311302 title "On the selective detection of duplex deoxyribonucleic acids by 2,1,3-benzothiadiazole fluorophores" @default.
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- W2133311302 doi "https://doi.org/10.1039/b919155k" @default.
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