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• W2133538560 endingPage "1155" @default.
• W2133538560 startingPage "1147" @default.
• W2133538560 abstract "Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) is a UV-B-specific signaling component that mediates low fluence, photomorphogenic responses to UV-B. It is required for UV-B-induced expression of the gene encoding the ELONGATED HYPOCOTYL5 (HY5) transcription factor. HY5 is a key effector of responses mediated by UVR8. In mature leaf tissue, HY5 transcript accumulation occurred rapidly in response to a brief UV-B treatment and no induction was observed in a uvr8 mutant over a broad range of UV wavelengths. In response to monochromatic light, maximal transcript accumulation occurred in wild-type plants at wavelengths 280-300 nm. HY5 transcript accumulation showed reciprocity between the fluence rate and duration of UV-B exposure, and on this basis conditions were chosen to generate an action spectrum for the UVR8 signaling pathway. Dose-response curves were produced for a range of UV wavelengths using 20 min exposure to UV and harvesting tissue 2 h after the start of illumination. Experiments using mutants defective in sinapate ester and flavonoid biosynthesis indicated that the presence of UV-absorbing compounds did not affect the construction of an action spectrum under the conditions employed. The action spectrum for the induction of HY5 by the UVR8 pathway showed a main peak at 280 nm with a smaller peak at 300 nm. The data are discussed in relation to the proposed mechanisms of UV-B photoreception." @default.
• W2133538560 created "2016-06-24" @default.
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• W2133538560 creator A5021814869 @default.
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• W2133538560 date "2009-09-01" @default.
• W2133538560 modified "2023-09-26" @default.
• W2133538560 title "UV-B Action Spectrum for UVR8-Mediated<i>HY5</i>Transcript Accumulation in Arabidopsis" @default.
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• W2133538560 doi "https://doi.org/10.1111/j.1751-1097.2009.00579.x" @default.
• W2133538560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19558421" @default.
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