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- W2133554518 endingPage "525" @default.
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- W2133554518 abstract "The cellular ESCRT (endosomal sorting complexes required for transport) pathway drives membrane constriction toward the cytosol and effects membrane fission during cytokinesis, endosomal sorting, and the release of many enveloped viruses, including the human immunodeficiency virus. A component of this pathway, the AAA ATPase Vps4, provides energy for pathway progression. Although it is established that Vps4 functions as an oligomer, subunit stoichiometry and other fundamental features of the functional enzyme are unclear. Here, we report that although some mutant Vps4 proteins form dodecameric assemblies, active wild-type Saccharomyces cerevisiae and Sulfolobus solfataricus Vps4 enzymes can form hexamers in the presence of ATP and ADP, as assayed by size-exclusion chromatography and equilibrium analytical ultracentrifugation. The Vta1p activator binds hexameric yeast Vps4p without changing the oligomeric state of Vps4p, implying that the active Vta1p-Vps4p complex also contains a single hexameric ring. Additionally, we report crystal structures of two different archaeal Vps4 homologs, whose structures and lattice interactions suggest a conserved mode of oligomerization. Disruption of the proposed hexamerization interface by mutagenesis abolished the ATPase activity of archaeal Vps4 proteins and blocked Vps4p function in S. cerevisiae. These data challenge the prevailing model that active Vps4 is a double-ring dodecamer, and argue that, like other type I AAA ATPases, Vps4 functions as a single ring with six subunits." @default.
- W2133554518 created "2016-06-24" @default.
- W2133554518 creator A5002864551 @default.
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- W2133554518 creator A5062562907 @default.
- W2133554518 creator A5086715753 @default.
- W2133554518 date "2014-02-01" @default.
- W2133554518 modified "2023-10-02" @default.
- W2133554518 title "The Oligomeric State of the Active Vps4 AAA ATPase" @default.
- W2133554518 cites W1501858161 @default.
- W2133554518 cites W1539796472 @default.
- W2133554518 cites W1965277349 @default.
- W2133554518 cites W1969219445 @default.
- W2133554518 cites W1972465158 @default.
- W2133554518 cites W1977346531 @default.
- W2133554518 cites W1981341553 @default.
- W2133554518 cites W1981515958 @default.
- W2133554518 cites W1982003972 @default.
- W2133554518 cites W1982606786 @default.
- W2133554518 cites W1982739808 @default.
- W2133554518 cites W1985747385 @default.
- W2133554518 cites W1988725811 @default.
- W2133554518 cites W1992752531 @default.
- W2133554518 cites W1993232578 @default.
- W2133554518 cites W1993871755 @default.
- W2133554518 cites W1994256471 @default.
- W2133554518 cites W1994286350 @default.
- W2133554518 cites W1998307755 @default.
- W2133554518 cites W2002374533 @default.
- W2133554518 cites W2003979167 @default.
- W2133554518 cites W2004130862 @default.
- W2133554518 cites W2009406517 @default.
- W2133554518 cites W2010958487 @default.
- W2133554518 cites W2019030289 @default.
- W2133554518 cites W2021105852 @default.
- W2133554518 cites W2024519032 @default.
- W2133554518 cites W2028904598 @default.
- W2133554518 cites W2031935325 @default.
- W2133554518 cites W2035503835 @default.
- W2133554518 cites W2038840577 @default.
- W2133554518 cites W2039695824 @default.
- W2133554518 cites W2040007466 @default.
- W2133554518 cites W2045968066 @default.
- W2133554518 cites W2046068788 @default.
- W2133554518 cites W2047854788 @default.
- W2133554518 cites W2059265460 @default.
- W2133554518 cites W2059494185 @default.
- W2133554518 cites W2059531322 @default.
- W2133554518 cites W2060380070 @default.
- W2133554518 cites W2060864992 @default.
- W2133554518 cites W2063103841 @default.
- W2133554518 cites W2063732204 @default.
- W2133554518 cites W2067665559 @default.
- W2133554518 cites W2067911054 @default.
- W2133554518 cites W2067933199 @default.
- W2133554518 cites W2068654171 @default.
- W2133554518 cites W2073158371 @default.
- W2133554518 cites W2078211289 @default.
- W2133554518 cites W2082768551 @default.
- W2133554518 cites W2092421259 @default.
- W2133554518 cites W2093442455 @default.
- W2133554518 cites W2094996600 @default.
- W2133554518 cites W2095578584 @default.
- W2133554518 cites W2095937726 @default.
- W2133554518 cites W2101002332 @default.
- W2133554518 cites W2101541375 @default.
- W2133554518 cites W2102706169 @default.
- W2133554518 cites W2105477665 @default.
- W2133554518 cites W2108921801 @default.
- W2133554518 cites W2112003767 @default.
- W2133554518 cites W2115907325 @default.
- W2133554518 cites W2118755520 @default.
- W2133554518 cites W2119794978 @default.
- W2133554518 cites W2120193453 @default.
- W2133554518 cites W2121431496 @default.
- W2133554518 cites W2121633863 @default.
- W2133554518 cites W2124449369 @default.
- W2133554518 cites W2125312640 @default.
- W2133554518 cites W2129247085 @default.
- W2133554518 cites W2129372332 @default.
- W2133554518 cites W2135130533 @default.
- W2133554518 cites W2137954479 @default.
- W2133554518 cites W2140690761 @default.
- W2133554518 cites W2142796125 @default.
- W2133554518 cites W2144081223 @default.
- W2133554518 cites W2144998676 @default.
- W2133554518 cites W2145823322 @default.
- W2133554518 cites W2153438089 @default.
- W2133554518 cites W2156242042 @default.
- W2133554518 cites W2166456692 @default.
- W2133554518 cites W2167531619 @default.
- W2133554518 cites W2170955251 @default.
- W2133554518 cites W4234287785 @default.
- W2133554518 cites W4249167162 @default.