FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133612430> ?p ?o ?g. }

• W2133612430 endingPage "84" @default.
• W2133612430 startingPage "78" @default.
• W2133612430 abstract "It is well known that hypothalamic endogenous opioid peptides (EOP) exert an inhibitory influence on LH release, and that a restraint on this inhibitory tone triggers preovulatory LHRH and LH hypersecretion. Recent evidence suggests that hypothalamic neuropeptide Y (NPY) is an important component of the neural circuitry that participates in induction of the LH surge. We have reported previously that blockade of EOP influence by the opioid receptor antagonist, naloxone (NAL), stimulated NPY accumulation in the median eminence (ME) in association with increased LH release in estradiol-17 beta-primed ovariectomized rats. To evaluate whether a restraint on the EOP system will result in an increase in NPY synthesis, the effects of NAL infusion on preproNPY messenger RNA (mRNA) levels in the medial basal hypothalamus were studied by solution hybridization/RNase protection assay and by in situ hybridization. NAL (2 mg/0.6 ml.h) or saline (SAL, 0.6 ml/h) was infused for 3 h (1100-1400 h) via an intrajugular cannula in estradiol-17 beta-primed ovariectomized rats. In accord with previous studies, NAL infusion significantly increased plasma LH levels at 1400 h. concomitant with this activation of LH release, NPY gene expression was also augmented. As compared with initial control levels at 1100 h, preproNPY mRNA levels in the medial basal hypothalamus increased at 1400 h in SAL (106%)- and NAL (202%)-infused rats, and at this time, preproNPY mRNA levels were significantly higher in NAL-infused rats than in SAL-infused rats. In situ hybridization studies showed that NAL infusion significantly increased the preproNPY mRNA signal at 1400 h mainly in the rostral and middle regions of the arcuate nucleus (ARC) as compared with that seen at 1100 and 1400 h in SAL-infused rats. To examine further the relationship between the NAL-induced increase in LH release and increase in NPY gene expression, the effects of NPY mRNA antisense oligonucleotides (oligos) on NPY levels in the ME-ARC and on plasma LH levels were studied in NAL-infused rats. In NAL-infused rats, intracerebroventricular administration of a NPY antisense oligo (25 micrograms/rat) at 1000, 1200, and 1300 h decreased NPY levels in the ME-ARC and blocked the increase in plasma LH levels at 1500 h, whereas control missense oligos had no effect. Collectively, these results show that a decrease in opioid influence rapidly augments NPY gene expression in a subpopulation of neurons in the ARC, and support the hypothesis that a disinhibition from opioid influence acutely promotes NPY synthesis and release, which is necessary for phasic LH discharge in rats." @default.
• W2133612430 created "2016-06-24" @default.
• W2133612430 creator A5007466426 @default.
• W2133612430 creator A5023409618 @default.
• W2133612430 creator A5028444807 @default.
• W2133612430 creator A5047037088 @default.
• W2133612430 creator A5084980664 @default.
• W2133612430 date "1996-01-01" @default.
• W2133612430 modified "2023-10-18" @default.
• W2133612430 title "Disinhibition from opioid influence augments hypothalamic neuropeptide Y (NPY) gene expression and pituitary luteinizing hormone release: effects of NPY messenger ribonucleic acid antisense oligodeoxynucleotides." @default.
• W2133612430 cites W115190129 @default.
• W2133612430 cites W1595497744 @default.
• W2133612430 cites W1970460118 @default.
• W2133612430 cites W1970956274 @default.
• W2133612430 cites W1971650902 @default.
• W2133612430 cites W1974371245 @default.
• W2133612430 cites W1977707793 @default.
• W2133612430 cites W1983263104 @default.
• W2133612430 cites W1988149499 @default.
• W2133612430 cites W1995765320 @default.
• W2133612430 cites W1999428413 @default.
• W2133612430 cites W2004222158 @default.
• W2133612430 cites W2005539247 @default.
• W2133612430 cites W2008660628 @default.
• W2133612430 cites W2009030749 @default.
• W2133612430 cites W2017828692 @default.
• W2133612430 cites W2021615559 @default.
• W2133612430 cites W2024942055 @default.
• W2133612430 cites W2028698544 @default.
• W2133612430 cites W2028953214 @default.
• W2133612430 cites W2030616360 @default.
• W2133612430 cites W2035923958 @default.
• W2133612430 cites W2036216347 @default.
• W2133612430 cites W2040754874 @default.
• W2133612430 cites W2044202413 @default.
• W2133612430 cites W2046628195 @default.
• W2133612430 cites W2057964271 @default.
• W2133612430 cites W2064050380 @default.
• W2133612430 cites W2065190205 @default.
• W2133612430 cites W2066218748 @default.
• W2133612430 cites W2067634836 @default.
• W2133612430 cites W2075081428 @default.
• W2133612430 cites W2079803201 @default.
• W2133612430 cites W2086163312 @default.
• W2133612430 cites W2088269503 @default.
• W2133612430 cites W2089683663 @default.
• W2133612430 cites W2141009915 @default.
• W2133612430 cites W2163815564 @default.
• W2133612430 cites W30257253 @default.
• W2133612430 cites W621515123 @default.
• W2133612430 cites W2531658907 @default.
• W2133612430 doi "https://doi.org/10.1210/endo.137.1.8536645" @default.
• W2133612430 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8536645" @default.
• W2133612430 hasPublicationYear "1996" @default.
• W2133612430 type Work @default.
• W2133612430 sameAs 2133612430 @default.
• W2133612430 citedByCount "19" @default.
• W2133612430 crossrefType "journal-article" @default.
• W2133612430 hasAuthorship W2133612430A5007466426 @default.
• W2133612430 hasAuthorship W2133612430A5023409618 @default.
• W2133612430 hasAuthorship W2133612430A5028444807 @default.
• W2133612430 hasAuthorship W2133612430A5047037088 @default.
• W2133612430 hasAuthorship W2133612430A5084980664 @default.
• W2133612430 hasBestOaLocation W21336124301 @default.
• W2133612430 hasConcept C104317684 @default.
• W2133612430 hasConcept C118303440 @default.
• W2133612430 hasConcept C126322002 @default.
• W2133612430 hasConcept C134018914 @default.
• W2133612430 hasConcept C150194340 @default.
• W2133612430 hasConcept C151077648 @default.
• W2133612430 hasConcept C170493617 @default.
• W2133612430 hasConcept C185592680 @default.
• W2133612430 hasConcept C2777003273 @default.
• W2133612430 hasConcept C2778575703 @default.
• W2133612430 hasConcept C2778894405 @default.
• W2133612430 hasConcept C2779064019 @default.
• W2133612430 hasConcept C2779067335 @default.
• W2133612430 hasConcept C2781063702 @default.
• W2133612430 hasConcept C55493867 @default.
• W2133612430 hasConcept C71315377 @default.
• W2133612430 hasConcept C71924100 @default.
• W2133612430 hasConcept C81439078 @default.
• W2133612430 hasConcept C86803240 @default.
• W2133612430 hasConcept C88016717 @default.
• W2133612430 hasConcept C93984277 @default.
• W2133612430 hasConceptScore W2133612430C104317684 @default.
• W2133612430 hasConceptScore W2133612430C118303440 @default.
• W2133612430 hasConceptScore W2133612430C126322002 @default.
• W2133612430 hasConceptScore W2133612430C134018914 @default.
• W2133612430 hasConceptScore W2133612430C150194340 @default.
• W2133612430 hasConceptScore W2133612430C151077648 @default.
• W2133612430 hasConceptScore W2133612430C170493617 @default.
• W2133612430 hasConceptScore W2133612430C185592680 @default.
• W2133612430 hasConceptScore W2133612430C2777003273 @default.
• W2133612430 hasConceptScore W2133612430C2778575703 @default.
• W2133612430 hasConceptScore W2133612430C2778894405 @default.
• W2133612430 hasConceptScore W2133612430C2779064019 @default.
• W2133612430 hasConceptScore W2133612430C2779067335 @default.

• next