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• W2133639964 abstract "<h3>Abstract</h3> The use of skin virome for human identification purposes offers a unique approach to instances where a viable and statistically relevant human DNA profile is unavailable. The human skin virome may act as an alternative DNA profile and/or an additional form of probative genetic material. To date, no study has attempted to investigate the human virome over a time series across various physical locations of the body to identify its potential as a tool for human identification. For this study, we set out to evaluate the stability, diversity, and individualization of the human skin virome. An additional goal was to identify viral signatures that can be used in conjunction with traditional forensic STR loci. In order to accomplish this, human virome metagenomes were collected and sequenced from 42 individuals at three anatomical locations (left hand, right hand, and scalp) across multiple collections periods over a 6-month window of time. Assembly dependent and independent bioinformatic approaches were employed, along with a database-based assessment, which resulted in three sets of stable putative viral markers. In total, with the three sets combined, 59 viral species and uncharacterized viral genome assemblies were identified as being significantly stable (<i>P</i>=5.3×10^-15). Viral diversity, based on presence or absence, is significantly different across subjects (<i>P</i><0.001). Here we demonstrate that not only is the human virome applicable to be used for human identification, but we have identified many viral signatures that can be used for forensic applications, thus providing a foundation to the novel field of forensic virology. <h3>Highlights</h3> Here we provide the largest human skin virome study, to date. Our study revealed novel diversity findings of high abundance for certain viral taxa, for example, the Cress-like DNA phages, that have not previously been characterized in human skin viral ecology studies. There were 59 putative human skin viral biomarkers suitable for human identification from the core stable human skin virome of 42 subjects. The putative markers we identified were significantly stable over a 6-month period of time within individuals and across three autosomal locations of left hand, right hand, and scalp. Diversity of profiles, based on the presence and absence of our putative marker data set, were significantly different across test subjects." @default.
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• W2133639964 date "2012-11-06" @default.
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• W2133639964 title "Changes in tobacco industry advertising around high schools in Greece following an outdoor advertising ban: a follow-up study: Table 1" @default.
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• W2133639964 doi "https://doi.org/10.1136/tobaccocontrol-2012-050518" @default.
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