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- W2133707490 abstract "Off-line sample prefractionations applied prior to biomarker discovery proteomics are options to enable more protein identifications and detect low-abundance proteins. This work compared five commercial methods efficiency to raw serum analysis using label-free proteomics. The variability of the protein quantities determined for each process was similar to the unprefractionated serum. A 49% increase in protein identifications and 12.2% of reliable quantification were obtained. A 61 times lower limit of protein quantitation was reached compared to protein concentrations observed in raw serum. The concentrations of detected proteins were confronted to estimated reference values." @default.
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- W2133707490 date "2015-12-01" @default.
- W2133707490 modified "2023-09-25" @default.
- W2133707490 title "Comparison of serum fractionation methods by data independent label-free proteomics" @default.
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- W2133707490 doi "https://doi.org/10.1016/j.euprot.2015.07.009" @default.
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