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• W2133711297 abstract "Abstract Elucidating patterns of root growth is essential for a better understanding of the functioning of plant‐dominated ecosystems. To this end, reliable and inexpensive methods are required to determine species compositions of root samples containing multiple species. Previous studies use a range of PCR‐based approaches, but none have examined a species pool greater than 10 or 30 when evaluating mixed and single species samples, respectively. We present a method that evaluates size differences in fluorescently labelled PCR amplicons (fluorescent fragment length polymorphism) of the trn L intron and the trn T‐ trn L and trn L‐ trn F intergenic spacers . Amplification success of the trn T‐ trn L spacer was limited, but variation in the trn L intron and the trn L‐ trn F spacer was sufficient to distinguish over 80% of the 95 species (97% of the 77 genera) evaluated from a diverse fescue grassland community. Moreover, we identified species known to be present in mixed samples of 4, 8, 12, and 16 species on average 82% of the time. However, this approach is sensitive to detecting species known to be absent (false positives) when using our key of 95 species. Comparing unknowns to a limited species pool ameliorates this problem, comparable to a researcher using prior knowledge of what species could be found in a sample to constrain the identification of species. Comparisons to other methods and future improvements are discussed. This method is efficient, cost‐ effective and broadly applicable to many ecosystems." @default.
• W2133711297 created "2016-06-24" @default.
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• W2133711297 date "2010-12-13" @default.
• W2133711297 modified "2023-10-13" @default.
• W2133711297 title "Molecular identification of roots from a grassland community using size differences in fluorescently labelled PCR amplicons of three cpDNA regions" @default.
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• W2133711297 doi "https://doi.org/10.1111/j.1755-0998.2010.02893.x" @default.
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