Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133762013> ?p ?o ?g. }
- W2133762013 endingPage "4858" @default.
- W2133762013 startingPage "4848" @default.
- W2133762013 abstract "ABSTRACT The diarrheal mechanisms in Aeromonas enteritis are not completely understood. In this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier function of monolayers of human intestinal cells (HT-29/B6). Ion secretion was determined as a short-circuit current (I SC ) of HT-29/B6 monolayers mounted in Ussing-type chambers. Transepithelial resistance (R t ) served as a measure of permeability. A diarrheal strain of Aeromonas hydrophila (strain Sb) added to the mucosal side of HT-29/B6 monolayers induced a significant I SC (39 ± 3 μA/cm 2 ) and decreased the R t to ∼10% of the initial value. A qualitatively identical response was obtained with sterile supernatant of strain Sb, and Aeromonas supernatant also induced a significant I SC in totally stripped human colon. Tracer flux and ion replacement studies revealed the I SC to be mainly accounted for by electrogenic Cl − secretion. Supernatant applied serosally completely abolished basal I SC . The supernatant-induced I SC was inhibited by the protein kinase C inhibitor chelerythrine, whereas a protein kinase A inhibitor (H8) and a Ca 2+ chelator (BAPTA-AM) had no effect. Physicochemical properties indicated that the supernatant's active compound was an aerolysin-related Aeromonas beta-hemolysin. Accordingly, identical I SC and R t responses were obtained with Escherichia coli lysates harboring the cloned beta-hemolysin gene from strain SB or the aerA gene encoding for aerolysin. Sequence comparison revealed a 64% homology between aerolysin and the beta-hemolysin cloned from Aeromonas sp. strain Sb. In conclusion, beta-hemolysin secreted by pathogenic aeromonads induces active Cl − secretion in the intestinal epithelium, possibly by channel insertion into the apical membrane and by activation of protein kinase C." @default.
- W2133762013 created "2016-06-24" @default.
- W2133762013 creator A5010631385 @default.
- W2133762013 creator A5031972677 @default.
- W2133762013 creator A5049462186 @default.
- W2133762013 creator A5051195358 @default.
- W2133762013 creator A5060356857 @default.
- W2133762013 creator A5061655044 @default.
- W2133762013 creator A5073700728 @default.
- W2133762013 creator A5081013332 @default.
- W2133762013 date "2004-08-01" @default.
- W2133762013 modified "2023-10-02" @default.
- W2133762013 title "<i>Aeromonas hydrophila</i> Beta-Hemolysin Induces Active Chloride Secretion in Colon Epithelial Cells (HT-29/B6)" @default.
- W2133762013 cites W134444516 @default.
- W2133762013 cites W1527047915 @default.
- W2133762013 cites W1527749437 @default.
- W2133762013 cites W1535284248 @default.
- W2133762013 cites W1590193061 @default.
- W2133762013 cites W1669518780 @default.
- W2133762013 cites W1830282454 @default.
- W2133762013 cites W1839795657 @default.
- W2133762013 cites W1883179014 @default.
- W2133762013 cites W1914389557 @default.
- W2133762013 cites W1964843189 @default.
- W2133762013 cites W1975583932 @default.
- W2133762013 cites W1991870265 @default.
- W2133762013 cites W1992740312 @default.
- W2133762013 cites W1994792016 @default.
- W2133762013 cites W1999543220 @default.
- W2133762013 cites W2009830520 @default.
- W2133762013 cites W2016776155 @default.
- W2133762013 cites W2025912735 @default.
- W2133762013 cites W2039570072 @default.
- W2133762013 cites W2049288843 @default.
- W2133762013 cites W2055190287 @default.
- W2133762013 cites W2057626594 @default.
- W2133762013 cites W2094556047 @default.
- W2133762013 cites W2096327263 @default.
- W2133762013 cites W2097562412 @default.
- W2133762013 cites W2099621876 @default.
- W2133762013 cites W2110174748 @default.
- W2133762013 cites W2117986587 @default.
- W2133762013 cites W2120158093 @default.
- W2133762013 cites W2145796643 @default.
- W2133762013 cites W2146768590 @default.
- W2133762013 cites W2158540015 @default.
- W2133762013 cites W2160572677 @default.
- W2133762013 cites W2170372540 @default.
- W2133762013 cites W2194164402 @default.
- W2133762013 cites W2287886505 @default.
- W2133762013 cites W2408432782 @default.
- W2133762013 cites W2412564573 @default.
- W2133762013 cites W2418287327 @default.
- W2133762013 cites W2419078717 @default.
- W2133762013 cites W4233922621 @default.
- W2133762013 doi "https://doi.org/10.1128/iai.72.8.4848-4858.2004" @default.
- W2133762013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/470692" @default.
- W2133762013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15271947" @default.
- W2133762013 hasPublicationYear "2004" @default.
- W2133762013 type Work @default.
- W2133762013 sameAs 2133762013 @default.
- W2133762013 citedByCount "29" @default.
- W2133762013 countsByYear W21337620132012 @default.
- W2133762013 countsByYear W21337620132016 @default.
- W2133762013 countsByYear W21337620132017 @default.
- W2133762013 countsByYear W21337620132019 @default.
- W2133762013 countsByYear W21337620132021 @default.
- W2133762013 countsByYear W21337620132022 @default.
- W2133762013 countsByYear W21337620132023 @default.
- W2133762013 crossrefType "journal-article" @default.
- W2133762013 hasAuthorship W2133762013A5010631385 @default.
- W2133762013 hasAuthorship W2133762013A5031972677 @default.
- W2133762013 hasAuthorship W2133762013A5049462186 @default.
- W2133762013 hasAuthorship W2133762013A5051195358 @default.
- W2133762013 hasAuthorship W2133762013A5060356857 @default.
- W2133762013 hasAuthorship W2133762013A5061655044 @default.
- W2133762013 hasAuthorship W2133762013A5073700728 @default.
- W2133762013 hasAuthorship W2133762013A5081013332 @default.
- W2133762013 hasBestOaLocation W21337620131 @default.
- W2133762013 hasConcept C104317684 @default.
- W2133762013 hasConcept C136139683 @default.
- W2133762013 hasConcept C153911025 @default.
- W2133762013 hasConcept C174319367 @default.
- W2133762013 hasConcept C2779441561 @default.
- W2133762013 hasConcept C49039625 @default.
- W2133762013 hasConcept C523546767 @default.
- W2133762013 hasConcept C54355233 @default.
- W2133762013 hasConcept C55493867 @default.
- W2133762013 hasConcept C60987743 @default.
- W2133762013 hasConcept C86803240 @default.
- W2133762013 hasConcept C89423630 @default.
- W2133762013 hasConceptScore W2133762013C104317684 @default.
- W2133762013 hasConceptScore W2133762013C136139683 @default.
- W2133762013 hasConceptScore W2133762013C153911025 @default.
- W2133762013 hasConceptScore W2133762013C174319367 @default.
- W2133762013 hasConceptScore W2133762013C2779441561 @default.
- W2133762013 hasConceptScore W2133762013C49039625 @default.
- W2133762013 hasConceptScore W2133762013C523546767 @default.