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• W2133784051 abstract "Virus-like particles (VLPs) have been employed for a number of nanometric applications because they self-assemble, exhibit a high degree of symmetry, and can be genetically and chemically modified. However, high symmetry does not allow for a single unique modification site on the VLP. Here, we demonstrate the co-expression of the cytotoxic A2 protein and the coat protein of the bacteriophage Qβ to form a nearly monodispersed population of novel VLPs. Cell-free protein synthesis allows for direct access and optimization of protein-synthesis and VLP-assembly. The A2 is shown to be incorporated at high efficiency, approaching a theoretical maximum of one A2 per VLP. This work demonstrates de novo production of a novel VLP, which contains a unique site that has the potential for future nanometric engineering applications. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012" @default.
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• W2133784051 date "2011-11-28" @default.
• W2133784051 modified "2023-09-24" @default.
• W2133784051 title "The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis" @default.
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• W2133784051 doi "https://doi.org/10.1002/btpr.744" @default.
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