Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133849196> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2133849196 abstract "Lubiprostone is a synthetic bicyclic fatty acid derivative of prostaglandin E1 (PGE1) used for chronic constipation. The best known action of lubiprostone is simulation of Cl- dependent fluid secretion. In a mouse model of the genetic disease cystic fibrosis, we previously showed that in vivo administration of lubiprostone resulted in greater mucus accumulation in the small intestine. The aim of this study was to directly test whether lubiprostone stimulates intestinal mucin release.Mucin release was measured by mounting segments (4-5 cm) of mouse proximal-mid small intestine in an organ bath, allowing access to the perfusate (luminal) and the bath (serosal) solutions. Nifedipine (10-6 M) and indomethacin (10-5 M) were included in all solutions to inhibit smooth muscle activity and endogenous prostaglandin production, respectively. The tissue was equilibrated under flow for 30 min, using the perfusate collected during the final 10 min of the equilibration period to measure unstimulated release rate. Stimulus was then added to either the perfusate or the bath and the perfusate was collected for another 30 min to measure the stimulated mucin release rate. Mucin in perfusates was quantified by periodic acid-Schiff's base dot-blot assay, using purified pig gastric mucin as a standard.When applied luminally at 1 μM lubiprostone was ineffective at stimulating mucin release. When added to the serosal solution, 1 μM lubiprostone stimulated mucin release to ~300% of the unstimulated rate. As a positive control, serosal 1 μM prostaglandin E2 increased mucin release to ~400% of the unstimulated rate.These results support the idea that lubiprostone has prostaglandin-like actions on the intestine, which includes stimulation of mucin release. Stimulation of mucin release by lubiprostone may be protective in gastrointestinal conditions where loss of mucus is believed to contribute to pathogenesis. Thus, in addition to chronic constipation, there is greater potential for the therapeutic applications of lubiprostone." @default.
- W2133849196 created "2016-06-24" @default.
- W2133849196 creator A5038163774 @default.
- W2133849196 date "2012-11-06" @default.
- W2133849196 modified "2023-10-09" @default.
- W2133849196 title "Lubiprostone stimulates small intestinal mucin release" @default.
- W2133849196 cites W1988070235 @default.
- W2133849196 cites W1996933969 @default.
- W2133849196 cites W2021521740 @default.
- W2133849196 cites W2101710638 @default.
- W2133849196 cites W2101851685 @default.
- W2133849196 cites W2110560266 @default.
- W2133849196 cites W2114567997 @default.
- W2133849196 cites W2139824857 @default.
- W2133849196 cites W2142781849 @default.
- W2133849196 cites W2155862827 @default.
- W2133849196 cites W2167909798 @default.
- W2133849196 cites W72129089 @default.
- W2133849196 doi "https://doi.org/10.1186/1471-230x-12-156" @default.
- W2133849196 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3523065" @default.
- W2133849196 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23130661" @default.
- W2133849196 hasPublicationYear "2012" @default.
- W2133849196 type Work @default.
- W2133849196 sameAs 2133849196 @default.
- W2133849196 citedByCount "20" @default.
- W2133849196 countsByYear W21338491962014 @default.
- W2133849196 countsByYear W21338491962015 @default.
- W2133849196 countsByYear W21338491962016 @default.
- W2133849196 countsByYear W21338491962017 @default.
- W2133849196 countsByYear W21338491962019 @default.
- W2133849196 countsByYear W21338491962020 @default.
- W2133849196 countsByYear W21338491962021 @default.
- W2133849196 countsByYear W21338491962022 @default.
- W2133849196 countsByYear W21338491962023 @default.
- W2133849196 crossrefType "journal-article" @default.
- W2133849196 hasAuthorship W2133849196A5038163774 @default.
- W2133849196 hasBestOaLocation W21338491961 @default.
- W2133849196 hasConcept C126322002 @default.
- W2133849196 hasConcept C134018914 @default.
- W2133849196 hasConcept C142724271 @default.
- W2133849196 hasConcept C179264091 @default.
- W2133849196 hasConcept C185592680 @default.
- W2133849196 hasConcept C18903297 @default.
- W2133849196 hasConcept C2777312293 @default.
- W2133849196 hasConcept C2780664492 @default.
- W2133849196 hasConcept C2781093762 @default.
- W2133849196 hasConcept C2781112942 @default.
- W2133849196 hasConcept C3017955636 @default.
- W2133849196 hasConcept C71924100 @default.
- W2133849196 hasConcept C86803240 @default.
- W2133849196 hasConcept C98274493 @default.
- W2133849196 hasConceptScore W2133849196C126322002 @default.
- W2133849196 hasConceptScore W2133849196C134018914 @default.
- W2133849196 hasConceptScore W2133849196C142724271 @default.
- W2133849196 hasConceptScore W2133849196C179264091 @default.
- W2133849196 hasConceptScore W2133849196C185592680 @default.
- W2133849196 hasConceptScore W2133849196C18903297 @default.
- W2133849196 hasConceptScore W2133849196C2777312293 @default.
- W2133849196 hasConceptScore W2133849196C2780664492 @default.
- W2133849196 hasConceptScore W2133849196C2781093762 @default.
- W2133849196 hasConceptScore W2133849196C2781112942 @default.
- W2133849196 hasConceptScore W2133849196C3017955636 @default.
- W2133849196 hasConceptScore W2133849196C71924100 @default.
- W2133849196 hasConceptScore W2133849196C86803240 @default.
- W2133849196 hasConceptScore W2133849196C98274493 @default.
- W2133849196 hasIssue "1" @default.
- W2133849196 hasLocation W21338491961 @default.
- W2133849196 hasLocation W21338491962 @default.
- W2133849196 hasLocation W21338491963 @default.
- W2133849196 hasLocation W21338491964 @default.
- W2133849196 hasOpenAccess W2133849196 @default.
- W2133849196 hasPrimaryLocation W21338491961 @default.
- W2133849196 hasRelatedWork W1759143983 @default.
- W2133849196 hasRelatedWork W1988343102 @default.
- W2133849196 hasRelatedWork W2016034363 @default.
- W2133849196 hasRelatedWork W2022912043 @default.
- W2133849196 hasRelatedWork W2056100207 @default.
- W2133849196 hasRelatedWork W2089781193 @default.
- W2133849196 hasRelatedWork W2127644594 @default.
- W2133849196 hasRelatedWork W2133849196 @default.
- W2133849196 hasRelatedWork W2748952813 @default.
- W2133849196 hasRelatedWork W2802658014 @default.
- W2133849196 hasVolume "12" @default.
- W2133849196 isParatext "false" @default.
- W2133849196 isRetracted "false" @default.
- W2133849196 magId "2133849196" @default.
- W2133849196 workType "article" @default.