FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133923024> ?p ?o ?g. }

• W2133923024 endingPage "438" @default.
• W2133923024 startingPage "432" @default.
• W2133923024 abstract "Penicillin amidases (PAs) from E. coli and A. faecalisare periplasmic enzymes that contain one tightly bound Ca2+ per molecule that does not directly participate in the enzymatic function. This ion may, however, be required for the maturation of the pre-pro-enzyme. The pro-enzyme of homologous PAs are translocated through the Tat-(E. coli PAEC) and Sec- (A. faecalis PAAF) transport systems, respectively. Cell fractionation, electrophoresis, immunoblotting, and activity staining demonstrated that Ca2+ binding is required for the membrane transport and maturation of the pro-enzyme to active enzyme. Pro-enzyme without Ca2+ was targeted to the membrane but not translocated. Influence of Ca2+ in medium and feed was studied for high cell density cultivations of E. coli expressing these enzymes. Without Ca2+ in the feed the synthesis of the pre-pro-enzyme was hardly influenced. At optimal Ca2+ content in the feed the active enzyme amount could be increased by 2 orders of magnitude up to 0.9 g/L (PAEC) and 2.3 g/L (PAAF) or 4% (PAEC) and 8% (PAAF) of the cell dry weight. The corresponding specific activities are 1700 U (PAEC) and 14000 U (PAAF) per gram cell dry weight, respectively. These values are higher than those published previously. Thus, for optimal yields of the studied and other extra- and periplasmic enzymes that require Ca2+ or other ions as cofactors for membrane transport and maturation, sufficient cofactor must be added in the feed." @default.
• W2133923024 created "2016-06-24" @default.
• W2133923024 creator A5005356575 @default.
• W2133923024 creator A5027602601 @default.
• W2133923024 creator A5031275669 @default.
• W2133923024 creator A5032719227 @default.
• W2133923024 creator A5049612559 @default.
• W2133923024 creator A5051060773 @default.
• W2133923024 creator A5051292695 @default.
• W2133923024 creator A5075027784 @default.
• W2133923024 date "2008-09-05" @default.
• W2133923024 modified "2023-10-18" @default.
• W2133923024 title "Ca2+ Is a Cofactor Required for Membrane Transport and Maturation and Is a Yield-Determining Factor in High Cell Density Penicillin Amidase Production" @default.
• W2133923024 cites W1558834418 @default.
• W2133923024 cites W1815415187 @default.
• W2133923024 cites W1834872237 @default.
• W2133923024 cites W1866575290 @default.
• W2133923024 cites W1883490532 @default.
• W2133923024 cites W1964675530 @default.
• W2133923024 cites W1965990486 @default.
• W2133923024 cites W1967286436 @default.
• W2133923024 cites W1969902013 @default.
• W2133923024 cites W1989455203 @default.
• W2133923024 cites W1994467973 @default.
• W2133923024 cites W1995305196 @default.
• W2133923024 cites W1996499870 @default.
• W2133923024 cites W1998908967 @default.
• W2133923024 cites W2000223531 @default.
• W2133923024 cites W2001586974 @default.
• W2133923024 cites W2009282816 @default.
• W2133923024 cites W2012359868 @default.
• W2133923024 cites W2015574272 @default.
• W2133923024 cites W2044430464 @default.
• W2133923024 cites W2047187744 @default.
• W2133923024 cites W2059494208 @default.
• W2133923024 cites W2069695110 @default.
• W2133923024 cites W2072166484 @default.
• W2133923024 cites W2073464264 @default.
• W2133923024 cites W2078303301 @default.
• W2133923024 cites W2079711255 @default.
• W2133923024 cites W2084432213 @default.
• W2133923024 cites W2096022379 @default.
• W2133923024 cites W2099131317 @default.
• W2133923024 cites W2100837269 @default.
• W2133923024 cites W2109627194 @default.
• W2133923024 cites W2112775535 @default.
• W2133923024 cites W2131587178 @default.
• W2133923024 cites W2131649995 @default.
• W2133923024 cites W2263785727 @default.
• W2133923024 cites W2324713756 @default.
• W2133923024 cites W4230781348 @default.
• W2133923024 cites W4250127988 @default.
• W2133923024 cites W4251469381 @default.
• W2133923024 cites W4378799554 @default.
• W2133923024 doi "https://doi.org/10.1021/bp049636a" @default.
• W2133923024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15801782" @default.
• W2133923024 hasPublicationYear "2008" @default.
• W2133923024 type Work @default.
• W2133923024 sameAs 2133923024 @default.
• W2133923024 citedByCount "21" @default.
• W2133923024 countsByYear W21339230242012 @default.
• W2133923024 countsByYear W21339230242013 @default.
• W2133923024 countsByYear W21339230242014 @default.
• W2133923024 countsByYear W21339230242015 @default.
• W2133923024 countsByYear W21339230242017 @default.
• W2133923024 countsByYear W21339230242018 @default.
• W2133923024 crossrefType "journal-article" @default.
• W2133923024 hasAuthorship W2133923024A5005356575 @default.
• W2133923024 hasAuthorship W2133923024A5027602601 @default.
• W2133923024 hasAuthorship W2133923024A5031275669 @default.
• W2133923024 hasAuthorship W2133923024A5032719227 @default.
• W2133923024 hasAuthorship W2133923024A5049612559 @default.
• W2133923024 hasAuthorship W2133923024A5051060773 @default.
• W2133923024 hasAuthorship W2133923024A5051292695 @default.
• W2133923024 hasAuthorship W2133923024A5075027784 @default.
• W2133923024 hasConcept C104317684 @default.
• W2133923024 hasConcept C181199279 @default.
• W2133923024 hasConcept C185592680 @default.
• W2133923024 hasConcept C197957613 @default.
• W2133923024 hasConcept C201663137 @default.
• W2133923024 hasConcept C2777451570 @default.
• W2133923024 hasConcept C28212737 @default.
• W2133923024 hasConcept C33633552 @default.
• W2133923024 hasConcept C547475151 @default.
• W2133923024 hasConcept C55493867 @default.
• W2133923024 hasConcept C57409179 @default.
• W2133923024 hasConcept C65588867 @default.
• W2133923024 hasConcept C86803240 @default.
• W2133923024 hasConceptScore W2133923024C104317684 @default.
• W2133923024 hasConceptScore W2133923024C181199279 @default.
• W2133923024 hasConceptScore W2133923024C185592680 @default.
• W2133923024 hasConceptScore W2133923024C197957613 @default.
• W2133923024 hasConceptScore W2133923024C201663137 @default.
• W2133923024 hasConceptScore W2133923024C2777451570 @default.
• W2133923024 hasConceptScore W2133923024C28212737 @default.
• W2133923024 hasConceptScore W2133923024C33633552 @default.
• W2133923024 hasConceptScore W2133923024C547475151 @default.
• W2133923024 hasConceptScore W2133923024C55493867 @default.

• next