Matches in SemOpenAlex for { <https://semopenalex.org/work/W2133955734> ?p ?o ?g. }
- W2133955734 endingPage "129" @default.
- W2133955734 startingPage "118" @default.
- W2133955734 abstract "Immunohistochemical studies previously revealed the presence of the peptide transmitter N-acetylaspartylglutamate (NAAG) in spinal motor neurons, axons and presumptive neuromuscular junctions (NMJs). At synapses in the central nervous system, NAAG has been shown to activate the type 3 metabotropic glutamate receptor (mGluR3) and is inactivated by an extracellular peptidase, glutamate carboxypeptidase II. The present study tested the hypothesis that NAAG meets the criteria for classification as a co-transmitter at the vertebrate NMJ. Confocal microscopy confirmed the presence of NAAG immunoreactivity and extended the resolution of the peptide's location in the lizard (Anolis carolinensis) NMJ. NAAG was localised to a presynaptic region immediately adjacent to postsynaptic acetylcholine receptors. NAAG was depleted by potassium-induced depolarisation and by electrical stimulation of motor axons. The NAAG receptor, mGluR3, was localised to the presynaptic terminal consistent with NAAG's demonstrated role as a regulator of synaptic release at central synapses. In contrast, glutamate receptors, type 2 metabotropic glutamate receptor (mGluR2) and N-methyl-d-aspartate, were closely associated with acetylcholine receptors in the postsynaptic membrane. Glutamate carboxypeptidase II, the NAAG-inactivating enzyme, was identified exclusively in perisynaptic glial cells. This localisation was confirmed by the loss of immunoreactivity when these cells were selectively eliminated. Finally, electrophysiological studies showed that exogenous NAAG inhibited evoked neurotransmitter release by activating a group II metabotropic glutamate receptor (mGluR2 or mGluR3). Collectively, these data support the conclusion that NAAG is a co-transmitter at the vertebrate NMJ." @default.
- W2133955734 created "2016-06-24" @default.
- W2133955734 creator A5000676250 @default.
- W2133955734 creator A5010394870 @default.
- W2133955734 creator A5035103060 @default.
- W2133955734 creator A5052735717 @default.
- W2133955734 creator A5068813624 @default.
- W2133955734 creator A5089935074 @default.
- W2133955734 date "2012-11-08" @default.
- W2133955734 modified "2023-09-30" @default.
- W2133955734 title "Immunohistological and electrophysiological evidence that<i>N</i>-acetylaspartylglutamate is a co-transmitter at the vertebrate neuromuscular junction" @default.
- W2133955734 cites W1494565585 @default.
- W2133955734 cites W1508185815 @default.
- W2133955734 cites W1528907365 @default.
- W2133955734 cites W1580481741 @default.
- W2133955734 cites W1729884812 @default.
- W2133955734 cites W1907987546 @default.
- W2133955734 cites W1957159594 @default.
- W2133955734 cites W1973661207 @default.
- W2133955734 cites W1975907304 @default.
- W2133955734 cites W1977109693 @default.
- W2133955734 cites W1977978573 @default.
- W2133955734 cites W1989264219 @default.
- W2133955734 cites W1989396363 @default.
- W2133955734 cites W1995830387 @default.
- W2133955734 cites W1996259599 @default.
- W2133955734 cites W1997008326 @default.
- W2133955734 cites W1997503854 @default.
- W2133955734 cites W1998400056 @default.
- W2133955734 cites W2002901235 @default.
- W2133955734 cites W2006222347 @default.
- W2133955734 cites W2006276531 @default.
- W2133955734 cites W2006814415 @default.
- W2133955734 cites W2009229844 @default.
- W2133955734 cites W2011505468 @default.
- W2133955734 cites W2015218703 @default.
- W2133955734 cites W2017259177 @default.
- W2133955734 cites W2018436079 @default.
- W2133955734 cites W2024787731 @default.
- W2133955734 cites W2034811993 @default.
- W2133955734 cites W2038415660 @default.
- W2133955734 cites W2045376318 @default.
- W2133955734 cites W2049638035 @default.
- W2133955734 cites W2050416880 @default.
- W2133955734 cites W2055476559 @default.
- W2133955734 cites W2057341719 @default.
- W2133955734 cites W2062710304 @default.
- W2133955734 cites W2066244859 @default.
- W2133955734 cites W2074688705 @default.
- W2133955734 cites W2078051775 @default.
- W2133955734 cites W2081314299 @default.
- W2133955734 cites W2082590622 @default.
- W2133955734 cites W2083085713 @default.
- W2133955734 cites W2087566431 @default.
- W2133955734 cites W2088126293 @default.
- W2133955734 cites W2093566215 @default.
- W2133955734 cites W2094241937 @default.
- W2133955734 cites W2095681824 @default.
- W2133955734 cites W2103170292 @default.
- W2133955734 cites W2111448138 @default.
- W2133955734 cites W2117497657 @default.
- W2133955734 cites W2120307371 @default.
- W2133955734 cites W2122579691 @default.
- W2133955734 cites W2126730087 @default.
- W2133955734 cites W2133352714 @default.
- W2133955734 cites W2153213393 @default.
- W2133955734 cites W2157530501 @default.
- W2133955734 cites W2167698799 @default.
- W2133955734 cites W4299343141 @default.
- W2133955734 doi "https://doi.org/10.1111/ejn.12027" @default.
- W2133955734 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3538924" @default.
- W2133955734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23134476" @default.
- W2133955734 hasPublicationYear "2012" @default.
- W2133955734 type Work @default.
- W2133955734 sameAs 2133955734 @default.
- W2133955734 citedByCount "27" @default.
- W2133955734 countsByYear W21339557342013 @default.
- W2133955734 countsByYear W21339557342014 @default.
- W2133955734 countsByYear W21339557342015 @default.
- W2133955734 countsByYear W21339557342016 @default.
- W2133955734 countsByYear W21339557342017 @default.
- W2133955734 countsByYear W21339557342018 @default.
- W2133955734 countsByYear W21339557342019 @default.
- W2133955734 countsByYear W21339557342020 @default.
- W2133955734 countsByYear W21339557342021 @default.
- W2133955734 countsByYear W21339557342022 @default.
- W2133955734 crossrefType "journal-article" @default.
- W2133955734 hasAuthorship W2133955734A5000676250 @default.
- W2133955734 hasAuthorship W2133955734A5010394870 @default.
- W2133955734 hasAuthorship W2133955734A5035103060 @default.
- W2133955734 hasAuthorship W2133955734A5052735717 @default.
- W2133955734 hasAuthorship W2133955734A5068813624 @default.
- W2133955734 hasAuthorship W2133955734A5089935074 @default.
- W2133955734 hasBestOaLocation W21339557342 @default.
- W2133955734 hasConcept C121608353 @default.
- W2133955734 hasConcept C134018914 @default.
- W2133955734 hasConcept C169760540 @default.
- W2133955734 hasConcept C170493617 @default.