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• W2133958794 endingPage "2828" @default.
• W2133958794 startingPage "2819" @default.
• W2133958794 abstract "Abstract In mammalian cell culture producing therapeutic proteins, one of the important challenges is the use of several complex raw materials whose compositional variability is relatively high and their influences on cell culture is poorly understood. Under these circumstances, application of spectroscopic techniques combined with chemometrics can provide fast, simple, and non‐destructive ways to evaluate raw material quality, leading to more consistent cell culture performance. In this study, a comprehensive data fusion strategy of combining multiple spectroscopic techniques is investigated for the prediction of raw material quality in mammalian cell culture. To achieve this purpose, four different spectroscopic techniques of near‐infrared, Raman, 2D fluorescence, and X‐ray fluorescence spectra were employed for comprehensive characterization of soy hydrolysates which are commonly used as supplements in culture media. First, the different spectra were compared separately in terms of their prediction capability. Then, ensemble partial least squares (EPLS) was further employed by combining all of these spectral datasets in order to produce a more accurate estimation of raw material properties, and compared with other data fusion techniques. The results showed that data fusion models based on EPLS always exhibit best prediction accuracy among all the models including individual spectroscopic methods, demonstrating the synergetic effects of data fusion in characterizing the raw material quality. Biotechnol. Bioeng. 2012; 109: 2819–2828. © 2012 Wiley Periodicals, Inc." @default.
• W2133958794 created "2016-06-24" @default.
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• W2133958794 date "2012-05-28" @default.
• W2133958794 modified "2023-10-12" @default.
• W2133958794 title "Data fusion-based assessment of raw materials in mammalian cell culture" @default.
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• W2133958794 doi "https://doi.org/10.1002/bit.24548" @default.
• W2133958794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22566239" @default.
• W2133958794 hasPublicationYear "2012" @default.
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