FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134015622> ?p ?o ?g. }

• W2134015622 endingPage "483" @default.
• W2134015622 startingPage "473" @default.
• W2134015622 abstract "Tyrosine phosphorylation of multiple platelet proteins is stimulated by thrombin and other agonists that cause platelet aggregation and secretion. The phosphorylation of a subset of these proteins, including a protein tyrosine kinase, pp125FAK, is dependent on the platelet aggregation that follows fibrinogen binding to integrin alpha IIb beta 3. In this report, we examined whether fibrinogen binding, per se, triggers a process of tyrosine phosphorylation in the absence of exogenous agonists. Binding of soluble fibrinogen was induced with Fab fragments of an anti-beta 3 antibody (anti-LIBS6) that directly exposes the fibrinogen binding site in alpha IIb beta3. Proteins of 50-68 KD and 140 kD became phosphorylated on tyrosine residues in a fibrinogen-dependent manner. This response did not require prostaglandin synthesis, an increase in cytosolic free calcium, platelet aggregation or granule secretion, nor was it associated with tyrosine phosphorylation of pp125FAK. Tyrosine phosphorylation of the 50-68-kD and 140-kD proteins was also observed when (a) fibrinogen binding was stimulated by agonists such as epinephrine, ADP, or thrombin instead of by anti-LIBS6; (b) fragment X, a dimeric plasmin-derived fragment of fibrinogen was used instead of fibrinogen; or (c) alpha IIb beta 3 complexes were cross-linked by antibodies, even in the absence of fibrinogen. In contrast, no tyrosine phosphorylation was observed when the ligand consisted of monomeric cell recognition peptides derived from fibrinogen (RGDS or gamma 400-411). Fibrinogen-dependent tyrosine phosphorylation was inhibited by cytochalasin D. These studies demonstrate that fibrinogen binding to alpha IIb beta 3 initiates a process of tyrosine phosphorylation that precedes platelet aggregation and the phosphorylation of pp125FAK. This reaction may depend on the oligomerization of integrin receptors and on the state of actin polymerization, organizational processes that may juxtapose tyrosine kinases with their substrates." @default.
• W2134015622 created "2016-06-24" @default.
• W2134015622 creator A5011634465 @default.
• W2134015622 creator A5032907445 @default.
• W2134015622 creator A5039985167 @default.
• W2134015622 creator A5043845770 @default.
• W2134015622 creator A5047445350 @default.
• W2134015622 creator A5060459487 @default.
• W2134015622 date "1993-07-15" @default.
• W2134015622 modified "2023-10-11" @default.
• W2134015622 title "Adhesive ligand binding to integrin alpha IIb beta 3 stimulates tyrosine phosphorylation of novel protein substrates before phosphorylation of pp125FAK" @default.
• W2134015622 cites W133668382 @default.
• W2134015622 cites W1489612807 @default.
• W2134015622 cites W1509623942 @default.
• W2134015622 cites W1530177683 @default.
• W2134015622 cites W1542978449 @default.
• W2134015622 cites W1548240869 @default.
• W2134015622 cites W1549544590 @default.
• W2134015622 cites W1551479053 @default.
• W2134015622 cites W1553345337 @default.
• W2134015622 cites W1569078259 @default.
• W2134015622 cites W1595161640 @default.
• W2134015622 cites W1598287593 @default.
• W2134015622 cites W1601529928 @default.
• W2134015622 cites W1605746073 @default.
• W2134015622 cites W1605961154 @default.
• W2134015622 cites W1632662791 @default.
• W2134015622 cites W1656991593 @default.
• W2134015622 cites W1908892780 @default.
• W2134015622 cites W1924175076 @default.
• W2134015622 cites W1966286149 @default.
• W2134015622 cites W1971557011 @default.
• W2134015622 cites W1981438065 @default.
• W2134015622 cites W1981810445 @default.
• W2134015622 cites W1982641617 @default.
• W2134015622 cites W1983198197 @default.
• W2134015622 cites W1983212899 @default.
• W2134015622 cites W1986586769 @default.
• W2134015622 cites W1995282093 @default.
• W2134015622 cites W2000808409 @default.
• W2134015622 cites W2002866097 @default.
• W2134015622 cites W2009967151 @default.
• W2134015622 cites W2010265636 @default.
• W2134015622 cites W2022407020 @default.
• W2134015622 cites W2025018535 @default.
• W2134015622 cites W2026327508 @default.
• W2134015622 cites W2029141750 @default.
• W2134015622 cites W2029231027 @default.
• W2134015622 cites W2032359283 @default.
• W2134015622 cites W2036145275 @default.
• W2134015622 cites W203618348 @default.
• W2134015622 cites W2043117244 @default.
• W2134015622 cites W2049311704 @default.
• W2134015622 cites W2051942264 @default.
• W2134015622 cites W2053014663 @default.
• W2134015622 cites W2056770464 @default.
• W2134015622 cites W2059188737 @default.
• W2134015622 cites W2062576661 @default.
• W2134015622 cites W2066560193 @default.
• W2134015622 cites W2081223071 @default.
• W2134015622 cites W2084162464 @default.
• W2134015622 cites W2108674983 @default.
• W2134015622 cites W2110785919 @default.
• W2134015622 cites W2124990648 @default.
• W2134015622 cites W2148129851 @default.
• W2134015622 cites W2148849095 @default.
• W2134015622 cites W2152133730 @default.
• W2134015622 cites W2162700552 @default.
• W2134015622 cites W2162761526 @default.
• W2134015622 cites W2403954747 @default.
• W2134015622 cites W2407299336 @default.
• W2134015622 cites W2409604495 @default.
• W2134015622 cites W2426032331 @default.
• W2134015622 cites W5386695 @default.
• W2134015622 doi "https://doi.org/10.1083/jcb.122.2.473" @default.
• W2134015622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2119653" @default.
• W2134015622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7686553" @default.
• W2134015622 hasPublicationYear "1993" @default.
• W2134015622 type Work @default.
• W2134015622 sameAs 2134015622 @default.
• W2134015622 citedByCount "186" @default.
• W2134015622 countsByYear W21340156222012 @default.
• W2134015622 countsByYear W21340156222014 @default.
• W2134015622 countsByYear W21340156222015 @default.
• W2134015622 countsByYear W21340156222016 @default.
• W2134015622 countsByYear W21340156222017 @default.
• W2134015622 countsByYear W21340156222019 @default.
• W2134015622 countsByYear W21340156222021 @default.
• W2134015622 countsByYear W21340156222022 @default.
• W2134015622 crossrefType "journal-article" @default.
• W2134015622 hasAuthorship W2134015622A5011634465 @default.
• W2134015622 hasAuthorship W2134015622A5032907445 @default.
• W2134015622 hasAuthorship W2134015622A5039985167 @default.
• W2134015622 hasAuthorship W2134015622A5043845770 @default.
• W2134015622 hasAuthorship W2134015622A5047445350 @default.
• W2134015622 hasAuthorship W2134015622A5060459487 @default.
• W2134015622 hasBestOaLocation W21340156221 @default.
• W2134015622 hasConcept C11960822 @default.

• next