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• W2134080276 endingPage "4392" @default.
• W2134080276 startingPage "4381" @default.
• W2134080276 abstract "Many proteins that contain a carboxyl-terminal CaaX sequence motif, including Ras and yeast a-factor, undergo a series of sequential posttranslational processing steps. Following the initial prenylation of the cysteine, the three C-terminal amino acids are proteolytically removed, and the newly formed prenylcysteine is carboxymethylated. The specific amino acids that comprise the CaaX sequence influence whether the protein can be prenylated and proteolyzed. In this study, we evaluated processing of a-factor variants with all possible single amino acid substitutions at either the a1, the a2, or the X position of the a-factor Ca1a2X sequence, CVIA. The substrate specificity of the two known yeast CaaX proteases, Afc1p and Rce1p, was investigated in vivo. Both Afc1p and Rce1p were able to proteolyze a-factor with A, V, L, I, C, or M at the a1 position, V, L, I, C, or M at the a2 position, or any amino acid at the X position that was acceptable for prenylation of the cysteine. Eight additional a-factor variants with a1 substitutions were proteolyzed by Rce1p but not by Afc1p. In contrast, Afc1p was able to proteolyze additional a-factor variants that Rce1p may not be able to proteolyze. In vitro assays indicated that farnesylation was compromised or undetectable for 11 a-factor variants that produced no detectable halo in the wild-type AFC1 RCE1 strain. The isolation of mutations in RCE1 that improved proteolysis of a-factor-CAMQ, indicated that amino acid substitutions E139K, F189L, and Q201R in Rce1p affected its substrate specificity." @default.
• W2134080276 created "2016-06-24" @default.
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• W2134080276 date "2000-06-01" @default.
• W2134080276 modified "2023-10-16" @default.
• W2134080276 title "The CaaX Proteases, Afc1p and Rce1p, Have Overlapping but Distinct Substrate Specificities" @default.
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• W2134080276 doi "https://doi.org/10.1128/mcb.20.12.4381-4392.2000" @default.
• W2134080276 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/85805" @default.
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